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使用自主研发的微生物群落对高密度聚乙烯和低密度聚乙烯进行比较生物降解

Comparative biodegradation of HDPE and LDPE using an indigenously developed microbial consortium.

作者信息

Satlewal Alok, Soni Ravindra, Zaidi Mgh, Shouche Yogesh, Goel Reeta

机构信息

Department of Microbiology, C.B.S.H., G.B. Pant University of Agriculture and Technology, Pantnagar-263145, India.

出版信息

J Microbiol Biotechnol. 2008 Mar;18(3):477-82.

Abstract

A variety of bacterial strains were isolated from waste disposal sites of Uttaranchal, India, and some from artificially developed soil beds containing maleic anhydride, glucose, and small pieces of polyethylene. Primary screening of isolates was done based on their ability to utilize high- and low-density polyethylenes (HDPE/LDPE) as a primary carbon source. Thereafter, a consortium was developed using potential strains. Furthermore, a biodegradation assay was carried out in 500-ml flasks containing minimal broth (250 ml) and HDPE/ LDPE at 5 mg/ml concentration. After incubation for two weeks, degraded samples were recovered through filtration and subsequent evaporation. Fourier transform infrared spectroscopy (FTIR) and simultaneous thermogravimetric-differential thermogravimetry-differential thermal analysis TG-DTG-DTA) were used to analyze these samples. Results showed that consortium-treated HDPE (considered to be more inert relative to LDPE) was degraded to a greater extent 22.41% weight loss) in comparison with LDPE (21.70% weight loss), whereas, in the case of untreated samples, weight loss was more for LDPE than HDPE (4.5% and 2.5%, respectively) at 400 degrees . Therefore, this study suggests that polyethylene could be degraded by utilizing microbial consortia in an eco-friendly manner.

摘要

从印度北阿坎德邦的废物处理场分离出了多种细菌菌株,还有一些是从含有马来酸酐、葡萄糖和小块聚乙烯的人工培育土壤层中分离出来的。根据分离菌株利用高密度和低密度聚乙烯(HDPE/LDPE)作为主要碳源的能力进行了初步筛选。此后,利用有潜力的菌株组建了一个混合菌群。此外,在装有500毫升基础肉汤(250毫升)和浓度为5毫克/毫升的HDPE/LDPE的500毫升烧瓶中进行了生物降解试验。培养两周后,通过过滤和随后的蒸发回收降解后的样品。使用傅里叶变换红外光谱(FTIR)和同步热重-微商热重-差示热分析(TG-DTG-DTA)对这些样品进行分析。结果表明,与LDPE(失重21.70%)相比,混合菌群处理的HDPE(相对于LDPE被认为更具惰性)降解程度更大(失重22.41%),而在未处理样品的情况下,在400摄氏度时,LDPE的失重比HDPE更多(分别为4.5%和2.5%)。因此,本研究表明,利用微生物混合菌群可以以生态友好的方式降解聚乙烯。

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