Nádvorník P, Rozhold O, Kolarík J, Ondra P, Závodný P, Hlustík P
Department of Experimental Neurology, Palacký University, Olomouc.
Acta Univ Palacki Olomuc Fac Med. 1991;130:125-9.
In clinical and experimental conditions, a biochemical equipment and a histological image of a fresh embryonic nervous tissue and a tissue conserved by deep freezing for transplantation purposes were examined. By repeated examination of neurotransmitters, the contents of dopamine and serotonin were found unchanged by freezing. In a human embryo, serotonin predominates over dopamine, while in chicken embryos, used for xeno-transplantations, it is vice versa. As for the microscopic structure of nervous tissue, no influence of kryopreservation for 4 to 14 days was found.
在临床和实验条件下,对用于移植目的的新鲜胚胎神经组织以及经深度冷冻保存的组织的生化设备和组织学图像进行了检查。通过对神经递质的反复检测,发现多巴胺和血清素的含量不受冷冻影响。在人类胚胎中,血清素的含量高于多巴胺,而在用于异种移植的鸡胚胎中则相反。至于神经组织的微观结构,未发现4至14天的低温保存有任何影响。