从埃及阿斯尤特市污染场地分离出的铜绿假单胞菌重金属抗性菌株的分离与鉴定

Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt.

作者信息

Hassan S H A, Abskharon R N N, El-Rab S M F Gad, Shoreit A A M

机构信息

University of Assiut, Faculty of Science, Botany Department, Assiut, Egypt.

出版信息

J Basic Microbiol. 2008 Jun;48(3):168-76. doi: 10.1002/jobm.200700338.

DOI:10.1002/jobm.200700338

PMID:18506899

Abstract

Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu(2+), Co(2+), Ni(2+), Zn(2+), Cr(3+), Cd(2+)and Pb(2+) were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni(2+) and Pb(2+) stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization.

摘要

从埃及阿斯尤特市的马拉赫运河废水中分离出66株假单胞菌属菌株，并检测了它们对重金属的耐受性。对其中一株菌株进行了多种金属抗性测试，发现其对镍和铅的抗性由分子量为27 Kb的质粒介导。它被鉴定为铜绿假单胞菌ASU 6a。其对Cu(2+)、Co(2+)、Ni(2+)、Zn(2+)、Cr(3+)、Cd(2+)和Pb(2+)的最低抑菌浓度（MIC）分别为6.3、5.9、6.8、9.2、5.8、4.4和3.1 mM。在Ni(2+)和Pb(2+)胁迫下测定了生长动力学和最大吸附容量。后两种重金属诱导钾离子外流，并被用作质膜透化的指标。