Silva Danielle C G, Yamanaka Naoki, Brogin Rodrigo L, Arias Carlos A A, Nepomuceno Alexandre L, Di Mauro Antônio O, Pereira Selma S, Nogueira Livia M, Passianotto André L L, Abdelnoor Ricardo V
Brazilian Agricultural Research Corporation-Embrapa Soybean, Caixa Postal 231, 86001-970 Londrina, PR, Brazil.
Theor Appl Genet. 2008 Jun;117(1):57-63. doi: 10.1007/s00122-008-0752-0. Epub 2008 Apr 8.
Asian soybean rust (ASR) is caused by the fungal pathogen Phakopsora pachyrhizi Sydow & Sydow. It was first identified in Brazil in 2001 and quickly infected soybean areas in several countries in South America. Primary efforts to combat this disease must involve the development of resistant cultivars. Four distinct genes that confer resistance against ASR have been reported: Rpp1, Rpp2, Rpp3, and Rpp4. However, no cultivar carrying any of those resistance loci has been released. The main objective of this study was to genetically map Rpp2 and Rpp4 resistance genes. Two F(2:3) populations, derived from the crosses between the resistant lines PI 230970 (Rpp2), PI 459025 (Rpp4) and the susceptible cultivar BRS 184, were used in this study. The mapping populations and parental lines were inoculated with a field isolate of P. pachyrhizi and evaluated for lesion type as resistant (RB lesions) or susceptible (TAN lesions). The mapping populations were screened with SSR markers, using the bulk segregant analysis (BSA) to expedite the identification of linked markers. Both resistance genes showed an expected segregation ratio for a dominant trait. This study allowed mapping Rpp2 and Rpp4 loci on the linkage groups J and G, respectively. The associated markers will be of great value on marker assisted selection for this trait.
亚洲大豆锈病（ASR）由真菌病原菌大豆锈菌（Phakopsora pachyrhizi Sydow & Sydow）引起。该病于2001年在巴西首次被发现，并迅速感染了南美洲几个国家的大豆种植区。对抗这种疾病的主要措施必须包括培育抗病品种。已报道了四个赋予对ASR抗性的不同基因：Rpp1、Rpp2、Rpp3和Rpp4。然而，尚未有携带任何这些抗性位点的品种被发布。本研究的主要目的是对Rpp2和Rpp4抗性基因进行遗传定位。本研究使用了两个F(2:3)群体，它们分别来源于抗性品系PI 230970（Rpp2）、PI 459025（Rpp4）与感病品种BRS 184之间的杂交。将这些定位群体和亲本系用大豆锈菌的一个田间分离株进行接种，并根据病斑类型评估为抗病（RB病斑）或感病（TAN病斑）。使用混合分离分析法（BSA），用SSR标记对定位群体进行筛选，以加快连锁标记的鉴定。两个抗性基因均表现出显性性状的预期分离比例。本研究分别将Rpp2和Rpp4位点定位到了连锁群J和G上。相关标记对于该性状的标记辅助选择将具有重要价值。
