Barros Luciane G, Avelino Bruna B, da Silva Danielle C G, Ferreira Everton G C, Castanho Fernanda M, Ferreira Marcio E, Lopes-Caitar Valeria S, Marin Silvana R R, Arias Carlos A A, de O N Lopes Ivani, Abdelnoor Ricardo V, Marcelino-Guimarães Francismar C
Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.
Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
Mol Breed. 2023 Feb 8;43(2):12. doi: 10.1007/s11032-023-01358-4. eCollection 2023 Feb.
Asian soybean rust (ASR), caused by the fungus , is the main disease affecting soybean in Brazil. This study aimed at investigating and mapping the resistance of the PI 594756 to , by using Bulked Segregant Analysis (BSA). The PI 594756 and the susceptible PI 594891 were crossed and the resulting and populations (208 and 1770 plants, respectively) were tested against ASR. Also, these PIs and differential varieties were tested against a panel of monosporic isolates. Plants presenting tan lesions were classified as susceptible () while plants presenting reddish-brown (RB) lesions were classified as resistant. DNA bulks were genotyped with Infinium BeadChips and the genomic region identified was further analyzed in the individuals with target GBS (tGBS). PI 594,56 presented a unique resistance profile compared to the differential varieties. The resistance was monogenic dominant; however, it was classified as incompletely dominant when quantitatively studied. Genetic and QTL mapping placed the PI 594756 gene between the genomic region located at 55,863,741 and 56,123,516 bp of chromosome 18. This position is slightly upstream mapping positions of (PI 200492) and (PI 594538A). Finally, we performed a haplotype analysis in a whole genomic sequencing-SNP database composed of Brazilian historical germplasm and sources of genes. We found SNPs that successfully differentiated the new PI 594756 allele from and sources. The haplotype identified can be used as a tool for marker-assisted selection (MAS).
The online version contains supplementary material available at 10.1007/s11032-023-01358-4.
由真菌引起的亚洲大豆锈病（ASR）是巴西影响大豆的主要病害。本研究旨在通过分离群体分析法（BSA）研究和定位PI 594756对[真菌名称未给出]的抗性。将PI 594756与感病的PI 594891杂交，所得的F1和F2群体（分别为208株和1770株）进行了抗ASR测试。此外，对这些PI和鉴别品种进行了一组单孢分离株的测试。出现棕褐色病斑的植株被分类为感病（S），而出现红棕色（RB）病斑的植株被分类为抗病。DNA混合池用Infinium BeadChips进行基因分型，在具有目标简化基因组测序（tGBS）的个体中进一步分析鉴定出的基因组区域。与鉴别品种相比，PI 594756呈现出独特的抗性谱。该抗性为单基因显性；然而，在进行定量研究时，它被分类为不完全显性。遗传和QTL定位将PI 594756基因定位在第18号染色体55,863,741至56,123,516 bp的基因组区域之间。该位置略位于[抗性基因名称未给出]（PI 200492）和[抗性基因名称未给出]（PI 594538A）图谱位置的上游。最后，我们在由巴西历史种质和[抗性基因名称未给出]基因来源组成的全基因组测序-SNP数据库中进行了单倍型分析。我们发现了能够成功区分新的PI 594756等位基因与[抗性基因名称未给出]和[抗性基因名称未给出]来源的SNP。鉴定出的单倍型可作为标记辅助选择（MAS）的工具。
在线版本包含可在10.1007/s11032-023-01358-4获取的补充材料。
