Olins Ada L, Zwerger Monika, Herrmann Harald, Zentgraf Hanswalter, Simon Amos J, Monestier Marc, Olins Donald E
Department of Biology, Bowdoin College, Brunswick, ME 04011, USA.
Eur J Cell Biol. 2008 May;87(5):279-90. doi: 10.1016/j.ejcb.2008.02.007. Epub 2008 Apr 8.
The human blood granulocyte (neutrophil) is adapted to find and destroy infectious agents. The nucleus of the human neutrophil has a segmented appearance, consisting of a linear or branched array of three or four lobes. Adequate levels of lamin B receptor (LBR) are necessary for differentiation of the lobulated nucleus. The levels of other components of the nuclear envelope may also be important for nuclear shape determination. In the present study, immunostaining and immunoblotting procedures explored the levels of various components of the nuclear envelope and heterochromatin, comparing freshly isolated human neutrophils with granulocytic forms of HL-60 cells, a tissue culture model system. In comparison to granulocytic HL-60 cells, blood neutrophil nuclear envelopes contain low-to-negligible amounts of LBR, lamins A/C, B1 and B2, LAP2beta and emerin. Surprisingly, a "mitotic" chromosome marker, H3(S10)phos, is elevated in neutrophil nuclei, compared to granulocytic HL-60 cells. Furthermore, neutrophil nuclei appear to be more fragile to methanol fixation, than observed with granulocytic HL-60 cells. Thus, the human neutrophil nucleus appears to be highly specialized, possessing a paucity of nuclear envelope-stabilizing proteins. In consequence, the neutrophil nucleus appears to be very malleable, supporting rapid migration through tight tissue spaces.
人类血液粒细胞（中性粒细胞）适于发现并消灭感染因子。人类中性粒细胞的细胞核呈分叶状，由三或四个叶的线性或分支状排列组成。足够水平的核纤层蛋白B受体（LBR）对于分叶状细胞核的分化是必需的。核膜其他成分的水平对于核形状的确定可能也很重要。在本研究中，免疫染色和免疫印迹程序探究了核膜和异染色质的各种成分的水平，将新鲜分离的人类中性粒细胞与HL-60细胞的粒细胞形式（一种组织培养模型系统）进行了比较。与粒细胞性HL-60细胞相比，血液中性粒细胞的核膜含有少量至可忽略不计的LBR、核纤层蛋白A/C、B1和B2、LAP2β和emerin。令人惊讶的是，与粒细胞性HL-60细胞相比，“有丝分裂”染色体标记H3(S10)phos在中性粒细胞核中升高。此外，与粒细胞性HL-60细胞相比，中性粒细胞核似乎对甲醇固定更脆弱。因此，人类中性粒细胞核似乎高度特化，缺乏核膜稳定蛋白。结果，中性粒细胞核似乎非常有延展性，支持其在紧密的组织间隙中快速迁移。
