Olins Ada L, Hoang Thanh V, Zwerger Monika, Herrmann Harald, Zentgraf Hanswalter, Noegel Angelika A, Karakesisoglou Iakowos, Hodzic Didier, Olins Donald E
Department of Biology, Bowdoin College, Brunswick, ME 04011, USA.
Eur J Cell Biol. 2009 Apr;88(4):203-14. doi: 10.1016/j.ejcb.2008.10.001. Epub 2008 Nov 18.
The major blood granulocyte (neutrophil) is rapidly recruited to sites of bacterial and fungal infections. It is a highly malleable cell, allowing it to squeeze out of blood vessels and migrate through tight tissue spaces. The human granulocyte nucleus is lobulated and exhibits a paucity of nuclear lamins, increasing its capability for deformation. The present study examined the existence of protein connections between the nuclear envelope and cytoskeletal elements (the LINC complex) in differentiated cell states (i.e. granulocytic, monocytic and macrophage) of the human leukemic cell line HL-60, as well as in human blood leukocytes. HL-60 granulocytes exhibited a deficiency of several LINC complex proteins (i.e. nesprin 1 giant, nesprin 2 giant, SUN1, plectin and vimentin); whereas, the macrophage state revealed nesprin 1 giant, plectin and vimentin. Both states possessed SUN2 in the nuclear envelope. Parallel differences were observed with some of the LINC complex proteins in isolated human blood leukocytes, including macrophage cells derived from blood monocytes. The present study documenting the paucity of LINC complex proteins in granulocytic forms, in combination with previous data on granulocyte nuclear shape and nuclear envelope composition, suggest the hypothesis that these adaptations evolved to facilitate granulocyte cellular malleability.
主要的血液粒细胞（中性粒细胞）会迅速被招募到细菌和真菌感染部位。它是一种具有高度可塑性的细胞，能够挤出血管并在紧密的组织间隙中迁移。人类粒细胞的细胞核呈分叶状，核纤层蛋白含量稀少，这增加了其变形能力。本研究检测了人类白血病细胞系HL-60的分化细胞状态（即粒细胞、单核细胞和巨噬细胞）以及人类血液白细胞中核膜与细胞骨架成分之间蛋白质连接（即LINC复合体）的存在情况。HL-60粒细胞表现出几种LINC复合体蛋白（即nesprin 1巨蛋白、nesprin 2巨蛋白、SUN1、网蛋白和波形蛋白）的缺乏；而巨噬细胞状态则显示有nesprin 1巨蛋白、网蛋白和波形蛋白。两种状态的核膜中均存在SUN2。在分离的人类血液白细胞中，包括源自血液单核细胞的巨噬细胞，也观察到了一些LINC复合体蛋白的类似差异。本研究记录了粒细胞形式中LINC复合体蛋白的缺乏，结合先前关于粒细胞核形状和核膜组成的数据，提出了这些适应性变化是为了促进粒细胞细胞可塑性而进化的假说。
