Sarkar Suchitra, Chakraborty Ranadhir
Department of Biotechnology, University of North Bengal, Siliguri, West Bengal, India.
FEMS Microbiol Lett. 2008 May;282(2):160-5. doi: 10.1111/j.1574-6968.2008.01080.x. Epub 2008 Apr 2.
Acinetobacter junii strain BB1A, a novel metal-tolerant bacterium, produced biofilm in the presence of added ions such as Ni(2+), AsO(2)(-), Cd(2+) and Hg(2+) on surfaces such as glass and polystyrene. Generation of a metal-sensitive and adhesion-deficient mutant by transposition of Tn5-mob in the A. junii genome has putatively confirmed the association of metal tolerance with the production of biofilm. The requirement of a critical cell density for biofilm formation and presence of acyl-homoserine lactone-like autoinducer molecules in the cell-free supernatant indicated the phenomenon of quorum sensing. Addition of a natural quorum-sensing inhibitor (garlic extract) or synthetic quorum-sensing inhibitor (4-nitro-pyridine oxide) significantly inhibited cell growth and biofilm formation in the presence of metal/metalloid ions.
琼氏不动杆菌菌株BB1A是一种新型耐金属细菌，在添加如Ni(2+)、AsO(2)(-)、Cd(2+)和Hg(2+)等离子的情况下，能在玻璃和聚苯乙烯等表面形成生物膜。通过在琼氏不动杆菌基因组中转座Tn5-mob产生的金属敏感且粘附缺陷的突变体，初步证实了金属耐受性与生物膜产生之间的关联。生物膜形成需要临界细胞密度，且无细胞上清液中存在酰基高丝氨酸内酯样自诱导分子，这表明存在群体感应现象。添加天然群体感应抑制剂（大蒜提取物）或合成群体感应抑制剂（4-硝基吡啶氧化物）可在金属/类金属离子存在的情况下显著抑制细胞生长和生物膜形成。
