Serrano M Concepción, Portolés M Teresa, Pagani Raffaella, de Guinoa Juan Sáez, Ruiz-Hernández Eduardo, Arcos Daniel, Vallet-Regí María
Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
Tissue Eng Part A. 2008 May;14(5):617-27. doi: 10.1089/tea.2007.0205.
A new kind of magnetic thermoseed for bone tissue engineering has been synthesized. The materials used are specially designed to restore bone tissue after tumor extirpation, because they exhibit bioactive behavior and the ability to act as thermoseeds for cancer treatment using hyperthermia. The L929 cell line of mouse fibroblasts has been used in a wide biocompatibility study concerning cell proliferation and morphology studies, mitochondrial function determination, lactate dehydrogenase measurement, and flow cytometry studies, including cell cycle analysis, cell size and complexity, and intracellular reactive oxygen species content. The results presented in this work indicate that these bioactive magnetic materials are highly biocompatible and show greater cell response for thermoseeds with a higher magnetic phase content. There were no significant alterations detected in the cell cycle, and the interaction between fibroblasts and the different mixtures did not induce significant apoptosis.
一种新型的用于骨组织工程的磁性热籽已被合成。所使用的材料经过特殊设计，用于在肿瘤切除后修复骨组织，因为它们具有生物活性行为以及作为热籽利用热疗治疗癌症的能力。小鼠成纤维细胞的L929细胞系已被用于一项广泛的生物相容性研究，该研究涉及细胞增殖和形态学研究、线粒体功能测定、乳酸脱氢酶测量以及流式细胞术研究，包括细胞周期分析、细胞大小和复杂性以及细胞内活性氧含量。这项工作中呈现的结果表明，这些生物活性磁性材料具有高度生物相容性，并且对于具有较高磁相含量的热籽显示出更大的细胞反应。在细胞周期中未检测到显著变化，并且成纤维细胞与不同混合物之间的相互作用未诱导显著的细胞凋亡。
