Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
J Biomed Mater Res A. 2012 Jan;100(1):64-71. doi: 10.1002/jbm.a.33229. Epub 2011 Oct 4.
The use of biomaterials as implantable thermoseeds under the action of an external magnetic field is a very interesting methodology to focus the heat into the target tumors as osteosarcoma. In this study, biocompatible and bioactive G15GC85 thermoseeds, tailored through the combination of sol-gel glasses (G) with a magnetic glass ceramic (GC), were used to induce hyperthermia on cultured human osteosarcoma cells after exposition to alternating magnetic field (MF, 100 kHz/200 Oe). G15GC85 magnetic glass-glass ceramic thermoseeds induced in vitro effective hyperthermia with drastic reduction in proliferation of human osteosarcoma Saos-2 cells and high increase of apoptotic cells after two 40 min consecutive sessions of MF. Deep cell morphology alterations were observed after this hyperthermic treatment, and the proteomic analysis revealed modification of gamma actin molecular properties related to cytoskeleton alterations. These results indicate that G15GC85 thermoseeds allow to induce in vitro effective hyperthermia on human osteosarcoma cells.
在外部磁场的作用下，将生物材料用作可植入热种子是一种非常有趣的方法，可以将热量集中到目标肿瘤（如骨肉瘤）中。在这项研究中，使用了生物相容性和生物活性的 G15GC85 热种子，通过溶胶-凝胶玻璃（G）与磁性玻璃陶瓷（GC）的组合来定制，以在暴露于交变磁场（MF，100 kHz/200 Oe）后诱导培养的人骨肉瘤细胞发生过热。G15GC85 磁性玻璃-玻璃陶瓷热种子在体外诱导了有效的过热，人骨肉瘤 Saos-2 细胞的增殖急剧减少，在两次连续的 MF 40 分钟疗程后凋亡细胞大量增加。过热治疗后观察到细胞形态的深刻变化，蛋白质组学分析表明与细胞骨架变化相关的γ肌动蛋白分子特性发生改变。这些结果表明，G15GC85 热种子可在体外有效地诱导人骨肉瘤细胞发生过热。
