Luppens S B I, Kara D, Bandounas L, Jonker M J, Wittink F R A, Bruning O, Breit T M, Ten Cate J M, Crielaard W
Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands.
Oral Microbiol Immunol. 2008 Jun;23(3):183-9. doi: 10.1111/j.1399-302X.2007.00409.x.
Our previous studies showed that Streptococcus mutans and Veillonella parvula dual-species biofilms have a different acid production profile and a higher resistance to chlorhexidine than their single-species counterparts. The aim of the current study was to test whether the susceptibility of S. mutans grown in the presence of V. parvula is also decreased when it is exposed to various other antimicrobials. Furthermore, the aim was to identify other changes in the physiology of S. mutans when V. parvula was present using transcriptomics.
Susceptibility to antimicrobials was assessed in killing experiments. Transcript levels in S. mutans were measured with the help of S. mutans microarrays.
When V. parvula was present, S. mutans showed an increase in survival after exposure to various antimicrobials. Furthermore, this co-existence altered the physiology of S. mutans. The expression of genes coding for proteins involved in amino acid synthesis, the signal recognition particle-translocation pathway, purine metabolism, intracellular polysaccharide synthesis, and protein synthesis all changed.
Growing in a biofilm together with a non-pathogenic bacterium like V. parvula changes the physiology of S. mutans, and gives this bacterium an advantage in surviving antimicrobial treatment. Thus, the study of pathogens implicated in polymicrobial diseases, such as caries and periodontitis, should be focused more on multispecies biofilms. In addition, the testing of susceptibility to currently used and new antimicrobials should be performed on a multispecies microbial community rather than with single pathogens.
我们之前的研究表明，变形链球菌和小韦荣球菌双物种生物膜具有不同的产酸模式，并且比它们的单物种生物膜对氯己定具有更高的耐受性。本研究的目的是测试在小韦荣球菌存在的情况下生长的变形链球菌在接触各种其他抗菌剂时其敏感性是否也会降低。此外，目的是使用转录组学来确定当小韦荣球菌存在时变形链球菌生理上的其他变化。
在杀菌实验中评估对抗菌剂的敏感性。借助变形链球菌微阵列测量变形链球菌中的转录水平。
当小韦荣球菌存在时，变形链球菌在接触各种抗菌剂后存活率增加。此外，这种共存改变了变形链球菌的生理状态。参与氨基酸合成、信号识别颗粒 - 转运途径、嘌呤代谢、细胞内多糖合成和蛋白质合成的蛋白质编码基因的表达均发生了变化。
与像小韦荣球菌这样的非致病性细菌一起在生物膜中生长会改变变形链球菌的生理状态，并使这种细菌在抗菌治疗中具有生存优势。因此，对涉及多微生物疾病（如龋齿和牙周炎）的病原体的研究应更多地关注多物种生物膜。此外，对当前使用的和新型抗菌剂的敏感性测试应在多物种微生物群落上进行，而不是针对单一病原体。
