Hammond B, Lemen J, Dudek R, Ward D, Jiang C, Nemeth M, Burns J
Monsanto Company, 800 North Lindbergh Blvd., St Louis, MO 63167, USA.
Food Chem Toxicol. 2006 Feb;44(2):147-60. doi: 10.1016/j.fct.2005.06.008. Epub 2005 Aug 9.
The results of a 90-day rat feeding study with YieldGard (YieldGard Rootworm Corn is a registered trademark of Monsanto Technology, LLC.) Rootworm corn (MON 863) grain that is protected against feeding damage caused by corn rootworm larvae are presented. Corn rootworm-protection was accomplished through the introduction of a cry3Bb1 coding sequence into the corn genome for in planta production of a modified Cry3Bb1 protein from Bacillus thuringiensis. Grain from MON 863 and its near isogenic control were separately formulated into rodent diets at levels of 11% and 33% (w/w) by Purina Mills, Inc. Additionally, six groups of rats were fed diets containing grain from different conventional (non-biotechnology-derived) reference varieties. The responses of rats fed diets containing MON 863 were compared to those of rats fed grain from conventional corn varieties. All diets were nutritionally balanced and conformed to Purina Mills, Inc. specifications for Certified LabDiet 5002. There were a total of 400 rats in the study divided into 10 groups of 20 rats/sex/group. Overall health, body weight gain, food consumption, clinical pathology parameters (hematology, blood chemistry, urinalysis), organ weights, gross and microscopic appearance of tissues were comparable between groups fed diets containing MON 863 and conventional corn varieties. This study complements extensive agronomic, compositional and farm animal feeding studies with MON 863 grain, confirming that it is as safe and nutritious as existing conventional corn varieties.
本文介绍了一项用抗虫玉米（先正达抗虫玉米是孟山都技术有限责任公司的注册商标）进行的为期90天的大鼠喂养研究结果。该抗虫玉米（MON 863）的谷粒可防止玉米根虫幼虫造成的取食损害。通过将cry3Bb1编码序列导入玉米基因组，在植物中产生来自苏云金芽孢杆菌的修饰Cry3Bb1蛋白，从而实现对玉米根虫的保护。孟山都公司（MON 863）及其近等基因对照的谷粒分别由普瑞纳磨坊公司（Purina Mills, Inc.）以11%和33%（w/w）的水平配制成啮齿动物饲料。此外，六组大鼠分别喂食含有不同常规（非生物技术衍生）参考品种谷粒的饲料。将喂食含MON 863饲料的大鼠的反应与喂食常规玉米品种谷粒的大鼠的反应进行比较。所有饲料营养均衡，符合普瑞纳磨坊公司认证实验室饲料5002的规格。该研究共有400只大鼠，分为10组，每组20只大鼠（雌雄各半）。喂食含MON 863饲料的组和喂食常规玉米品种饲料的组在总体健康、体重增加、食物消耗、临床病理参数（血液学、血液化学、尿液分析）、器官重量、组织的大体和微观外观方面具有可比性。这项研究补充了对MON 863谷粒进行的广泛农艺、成分和农场动物喂养研究，证实其与现有的常规玉米品种一样安全且营养丰富。
