Kim Hyeonjin, Booth Carmen J, Pinus Alexander B, Chen Pengyu, Lee Andrew, Qiu Maolin, Whitlock Mark, Murphy Philip S, Constable R Todd
Department of Diagnostic Radiology, Section of Comparative Medicine, Yale University, New Haven, Conn, USA.
Radiology. 2008 Jun;247(3):696-705. doi: 10.1148/radiol.2473070605. Epub 2008 Apr 10.
To prospectively evaluate magnetic resonance (MR) imaging for the characterization of liver fibrosis by estimating fat and extracellular matrix content and hepatic perfusion parameters in CCl(4)-treated rats.
The animal research protocol was approved by the Institutional Animal Care and Use Committee. Fifty-two rats (38 treated, 14 control) were included. A CCl(4) mixture was injected three times per week for 2-16 weeks. Fat-to-water ratios (FWRs) were calculated. Images were obtained with 12 saturation offset frequencies; magnetization transfer ratios (MTRs) were calculated. Distribution volume (DV), mean transit time (MTT), and portal fraction (PF) of blood inflow were calculated. For pairwise group comparisons, an unequal two-tailed Student t test was used. For pairwise correlations between variables, Pearson correlation coefficients were calculated. For multiple pairwise comparisons, Bonferroni correction was performed by adjusting the significance level (alpha).
FWR and DV were correlated with CCl(4) treatment duration from 0 through 8 weeks (r = 0.658, P < .001 and r = -0.664, P < .001, respectively; alpha = .010). PF and MTT were correlated with CCl(4) treatment duration from 0 through 16 weeks (r = -0.483, P = .002 and r = 0.414, P = .008, respectively; alpha = .010). DV was inversely correlated with FWR over the same period (r = -0.581, P < .001; alpha = .007). Fibrotic rats without cirrhosis had a higher FWR and lower DV and PF (P < .001, P < .001, and P = .004, respectively; alpha = .017) than control rats, and lower MTR, DV, and MTT (P = .014, .001, and .010, respectively; alpha = .017) than cirrhotic rats. Cirrhotic rats had a higher FWR and a lower PF (P < .001, alpha = .017) than control rats.
Magnetization transfer contrast is not a specific indicator of increased fibrosis in diseased liver; steatosis may influence some perfusion parameters.
通过评估四氯化碳处理大鼠的脂肪和细胞外基质含量以及肝脏灌注参数，前瞻性地评价磁共振（MR）成像对肝纤维化的特征描述。
动物研究方案经机构动物护理和使用委员会批准。纳入52只大鼠（38只处理组，14只对照组）。每周注射三次四氯化碳混合物，持续2至16周。计算脂肪与水的比率（FWR）。使用12个饱和偏移频率获取图像；计算磁化传递比率（MTR）。计算血液流入的分布容积（DV）、平均通过时间（MTT）和门静脉分数（PF）。对于成对组比较，使用不等双尾Student t检验。对于变量之间的成对相关性，计算Pearson相关系数。对于多个成对比较，通过调整显著性水平（α）进行Bonferroni校正。
FWR和DV与0至8周的四氯化碳处理持续时间相关（r分别为0.658，P <.001和r = -0.664，P <.001；α = 0.010）。PF和MTT与0至16周的四氯化碳处理持续时间相关（r分别为-0.483，P =.002和r = 0.414，P =.008；α = 0.010）。同期DV与FWR呈负相关（r = -0.581，P <.001；α = 0.007）。无肝硬化的纤维化大鼠比对照大鼠具有更高的FWR和更低的DV及PF（分别为P <.001，P <.001和P =.004；α = 0.017），并且比肝硬化大鼠具有更低的MTR、DV和MTT（分别为P =.014，.001和.010；α = 0.
