Svobodová Alena, Rambousková Jana, Walterová Daniela, Vostálová Jitka
Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
Arch Dermatol Res. 2008 Jun;300(5):225-33. doi: 10.1007/s00403-008-0850-5. Epub 2008 Apr 11.
In this study, the UVA (photo)protective activity of the phenolic fraction of L. caerulea fruits (PFLC) was assessed in human keratinocytes HaCaT. The keratinocytes were pre- or post-treated with PFLC (1-250 mg/l) and exposed to UVA irradiation (10-30 J/cm(2)). The results showed that both pre- and post-treatment with PFLC significantly suppressed UVA-induced ROS production, which was also revealed as a decrease in intracellular lipid peroxidation and elevation of reduced glutathione. Protection was concentration-dependent with a maximum at 50 mg/l. These results suggest that PFLC attenuates UVA-induced oxidative stress by reduction of ROS generation and ROS-mediated damage. For this reason, PFLC has potentially skin-protective functions against the deleterious effects of sunlight.
在本研究中，对欧洲越橘果实酚类组分（PFLC）在人角质形成细胞HaCaT中的UVA（光）保护活性进行了评估。角质形成细胞用PFLC（1 - 250 mg/l）进行预处理或后处理，然后暴露于UVA辐射（10 - 30 J/cm²）。结果表明，PFLC预处理和后处理均显著抑制UVA诱导的ROS产生，这也表现为细胞内脂质过氧化的减少和还原型谷胱甘肽的升高。保护作用呈浓度依赖性，在50 mg/l时达到最大值。这些结果表明，PFLC通过减少ROS生成和ROS介导的损伤来减轻UVA诱导的氧化应激。因此，PFLC对阳光的有害影响具有潜在的皮肤保护作用。
