Kawata Jyunichi, Kameda Munekazu, Miyazawa Mitsuo
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, 3-4-1 Kowakae, Higashiosaka-shi, Osaka, 577-8502, Japan.
J Nat Med. 2008 Apr;62(2):239-43. doi: 10.1007/s11418-007-0221-0. Epub 2008 Jan 11.
The composition of the volatile oil from Lithospermi Radix, the dried roots of Lithospermum erythrorhizon (Boraginaceae), has been investigated by capillary GC and GC-MS. To investigate the anti-inflammatory activity of the oil, in-vitro inhibition of ovine cyclooxygenase-1 and 2 (COX-1 and COX-2) activity by the oil was studied. Fifty-four components of the oil were identified, representing 92.74% of the oil. The main components were 2-methylbutanoic acid (21.50%), 3-methylbutanoic acid (12.61%), 2-methylpropanoic acid (8.99%), methyl linoleate (8.76%), methyl oleate (6.27%), methyl palmitate (6.06%), and 2-methyl-2-butenoic acid (5.74%). Highly selective COX-2 inhibition was observed; at 50 microg/ml the oil inhibited 38.8% of COX-2 activity.
采用毛细管气相色谱法（GC）和气相色谱-质谱联用法（GC-MS）对紫草科植物紫草干燥根的挥发油成分进行了研究。为研究该挥发油的抗炎活性，对其体外抑制绵羊环氧化酶-1和环氧化酶-2（COX-1和COX-2）活性进行了研究。该挥发油共鉴定出54种成分，占挥发油总量的92.74%。主要成分有2-甲基丁酸（21.50%）、3-甲基丁酸（12.61%）、2-甲基丙酸（8.99%）、亚油酸甲酯（8.76%）、油酸甲酯（6.27%）、棕榈酸甲酯（6.06%）和2-甲基-2-丁烯酸（5.74%）。观察到该挥发油对COX-2具有高度选择性抑制作用；在50μg/ml时，该挥发油抑制了38.8%的COX-2活性。
