Passos Giselle F, Fernandes Elizabeth S, da Cunha Fernanda M, Ferreira Juliano, Pianowski Luiz F, Campos Maria M, Calixto João B
Department of Pharmacology, Centre of Biological Sciences, Universidade Federal de Santa Catarina, 88049-900 Florianópolis, SC, Brazil.
J Ethnopharmacol. 2007 Mar 21;110(2):323-33. doi: 10.1016/j.jep.2006.09.032. Epub 2006 Nov 3.
The anti-inflammatory and anti-allergic effects of the essential oil of Cordia verbenacea (Boraginaceae) and some of its active compounds were evaluated. Systemic treatment with the essential oil of Cordia verbenacea (300-600mg/kg, p.o.) reduced carrageenan-induced rat paw oedema, myeloperoxidase activity and the mouse oedema elicited by carrageenan, bradykinin, substance P, histamine and platelet-activating factor. It also prevented carrageenan-evoked exudation and the neutrophil influx to the rat pleura and the neutrophil migration into carrageenan-stimulated mouse air pouches. Moreover, Cordia verbenacea oil inhibited the oedema caused by Apis mellifera venom or ovalbumin in sensitized rats and ovalbumin-evoked allergic pleurisy. The essential oil significantly decreased TNFalpha, without affecting IL-1beta production, in carrageenan-injected rat paws. Neither the PGE(2) formation after intrapleural injection of carrageenan nor the COX-1 or COX-2 activities in vitro were affected by the essential oil. Of high interest, the paw edema induced by carrageenan in mice was markedly inhibited by both sesquiterpenic compounds obtained from the essential oil: alpha-humulene and trans-caryophyllene (50mg/kg, p.o.). Collectively, the present results showed marked anti-inflammatory effects for the essential oil of Cordia verbenacea and some active compounds, probably by interfering with TNFalpha production. Cordia verbenacea essential oil or its constituents might represent new therapeutic options for the treatment of inflammatory diseases.
对马鞭草科植物光叶破布木精油及其部分活性化合物的抗炎和抗过敏作用进行了评估。光叶破布木精油（300 - 600mg/kg，口服）全身给药可减轻角叉菜胶诱导的大鼠足爪水肿、髓过氧化物酶活性，以及角叉菜胶、缓激肽、P物质、组胺和血小板活化因子引起的小鼠水肿。它还可预防角叉菜胶诱发的渗出以及中性粒细胞流入大鼠胸膜和迁移至角叉菜胶刺激的小鼠气囊。此外，光叶破布木精油可抑制致敏大鼠中由蜜蜂毒液或卵清蛋白引起的水肿以及卵清蛋白诱发的过敏性胸膜炎。在注射角叉菜胶的大鼠足爪中，该精油可显著降低肿瘤坏死因子α（TNFα）水平，而不影响白细胞介素-1β（IL-1β）的产生。胸膜内注射角叉菜胶后前列腺素E2（PGE2）的形成以及体外环氧化酶-1（COX-1）或环氧化酶-2（COX-2）的活性均不受该精油影响。值得关注的是，从该精油中获得的两种倍半萜化合物：α-葎草烯和反式石竹烯（50mg/kg，口服）均能显著抑制角叉菜胶诱导的小鼠足爪水肿。总体而言，目前的结果表明光叶破布木精油及其一些活性化合物具有显著的抗炎作用，可能是通过干扰TNFα的产生实现的。光叶破布木精油或其成分可能为炎症性疾病的治疗提供新的治疗选择。
