Bangsborg J M, Cianciotto N P, Hindersson P
Department of Bacteriology, University of Copenhagen, Denmark.
Infect Immun. 1991 Oct;59(10):3836-40. doi: 10.1128/iai.59.10.3836-3840.1991.
After the demonstration of analogs of the Legionella pneumophila macrophage infectivity potentiator (Mip) protein in other Legionella species, the Legionella micdadei mip gene was cloned and expressed in Escherichia coli. DNA sequence analysis of the L. micdadei mip gene contained in the plasmid pBA6004 revealed a high degree of homology (71%) to the L. pneumophila mip gene, with the predicted secondary structures of the two Mip proteins following the same pattern. Southern hybridization experiments, with the plasmid pBA6004 as the probe, suggested that the mip gene of L. micdadei has extensive homology with the mip-like genes of several Legionella species. Furthermore, amino acid sequence comparisons revealed significant homology to two eukaryotic proteins with isomerase activity (FK506-binding proteins).
在证明嗜肺军团菌巨噬细胞感染增强剂（Mip）蛋白的类似物存在于其他军团菌物种之后，米德戴军团菌的mip基因被克隆并在大肠杆菌中表达。对质粒pBA6004中所含米德戴军团菌mip基因的DNA序列分析显示，其与嗜肺军团菌mip基因具有高度同源性（71%），两种Mip蛋白的预测二级结构遵循相同模式。以质粒pBA6004为探针进行的Southern杂交实验表明，米德戴军团菌的mip基因与几种军团菌物种的mip样基因具有广泛同源性。此外，氨基酸序列比较显示，其与两种具有异构酶活性的真核蛋白（FK506结合蛋白）具有显著同源性。
