大肠杆菌调控基因mprA的核苷酸序列以及mprA缺陷型突变体的构建与鉴定。
Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants.
作者信息
del Castillo I, González-Pastor J E, San Millán J L, Moreno F
机构信息
Unidad de Genética Molecular, Hospital Ramón y Cajal, Carretera de Colmenar, Madrid, Spain.
出版信息
J Bacteriol. 1991 Jun;173(12):3924-9. doi: 10.1128/jb.173.12.3924-3929.1991.
Abstract
In high copy number, the Escherichia coli mprA gene reduces the synthesis of peptide microcins B17 and C7 (MccB17 and MccC7) and blocks the osmoinduction of the proU operon at the transcriptional level. mprA has been sequenced and shown to encode a polypeptide of 176 amino acids (Mr, 20,563). Insertion and deletion mutant mprA alleles were constructed and then transferred to the chromosome by allelic replacement. In these mutants, expression of two mcb-lacZ fusions was fivefold derepressed, indicating a negative regulatory role of mprA on the mcb operon (MccB17). In contrast, no effect of the MprA- mutations on the expression of mcc operon (MccC7) or on the osmoinduction of proU operon was observed.
摘要
在高拷贝数情况下，大肠杆菌mprA基因会降低肽微菌素B17和C7（MccB17和MccC7）的合成，并在转录水平上阻断proU操纵子的渗透诱导。mprA已被测序，显示编码一个由176个氨基酸组成的多肽（Mr，20,563）。构建了插入和缺失突变的mprA等位基因，然后通过等位基因替换将其转移到染色体上。在这些突变体中，两个mcb-lacZ融合蛋白的表达被解除抑制达五倍之多，表明mprA对mcb操纵子（MccB17）具有负调控作用。相比之下，未观察到MprA-突变对mcc操纵子（MccC7）的表达或proU操纵子的渗透诱导有任何影响。
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