García-Bustos J F, Pezzi N, Asensio C
Biochem Biophys Res Commun. 1984 Mar 15;119(2):779-85. doi: 10.1016/s0006-291x(84)80318-6.
Microcin 7 is an antibiotic peptide, produced and excreted to the culture medium by E. coli strains harboring the plasmid pRYC7. This peptide was extracted from the culture media by adsorbing it on octadecyl silica. It was purified by gel filtration on Sephadex G-25 and reverse phase high performance liquid chromatography. Its amino acid composition is the following: Ala (0.8), Arg (1.9), Asx (1.9), Gly (1.5), Met (0.8) and Thr (0.9). The purified peptide dose not react with ninhydrin and it is resistant to carboxypeptidase degradation, indicating that the molecule may be a cyclic or end-blocked oligopeptide.
微菌素7是一种抗生素肽，由携带质粒pRYC7的大肠杆菌菌株产生并分泌到培养基中。该肽通过吸附在十八烷基硅胶上从培养基中提取出来。它通过在葡聚糖凝胶G-25上进行凝胶过滤和反相高效液相色谱法进行纯化。其氨基酸组成如下：丙氨酸（0.8）、精氨酸（1.9）、天冬酰胺（1.9）、甘氨酸（1.5）、甲硫氨酸（0.8）和苏氨酸（0.9）。纯化后的肽与茚三酮不发生反应，并且对羧肽酶降解具有抗性，这表明该分子可能是一种环状或末端封闭的寡肽。
