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Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae.

作者信息

Lingner J, Kellermann J, Keller W

机构信息

Department of Cell Biology, University of Basel, Switzerland.

出版信息

Nature. 1991 Dec 12;354(6353):496-8. doi: 10.1038/354496a0.

DOI:10.1038/354496a0
PMID:1840648
Abstract

Poly(A) polymerase is essential for the maturation of messenger RNA, adding tracts of adenosine residues to the 3' end of precursor RNA generated by endonucleolytic cleavage. This mechanism of mRNA 3' processing seems to be similar in yeast and in higher eucaryotes, although there are differences in the recognition signals in the pre-mRNA. Here we describe the cloning of the gene for yeast poly(A) polymerase. The enzyme is encoded by a single and essential gene located near the centromere on the left arm of chromosome 11. Poly(A) polymerase purified from recombinant Escherichia coli has the same physical and biochemical properties as the yeast enzyme. The yeast poly(A) polymerase shares features of sequence with its mammalian homologue.

摘要

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