Raabe T, Bollum F J, Manley J L
Department of Biological Sciences, Columbia University, New York, New York 10027.
Nature. 1991 Sep 19;353(6341):229-34. doi: 10.1038/353229a0.
Poly(A) polymerase has a critical role in the synthesis of messenger RNA in eukaryotic cells. The isolation and characterization of complementary DNAs encoding bovine poly(A) polymerase is described here. The predicted sequences of the mRNA and protein reveal features that provide insights into how the enzyme functions and how it might be regulated. Poly(A) polymerase expressed from a cloned cDNA is fully functional in in vitro assays, and mutational analyses have identified a putative regulatory domain that enhances, but is not essential for, activity.
