使用Dentocult SM变链菌试纸法检测油拔法对菌斑和唾液中变形链球菌数量的影响：一项随机、对照、三盲研究。
Effect of oil pulling on Streptococcus mutans count in plaque and saliva using Dentocult SM Strip mutans test: a randomized, controlled, triple-blind study.
作者信息
Asokan S, Rathan J, Muthu M S, Rathna Prabhu V, Emmadi P
机构信息
Department of Pediatric Dentistry, Meenakshi Ammal Dental College, Chennai, Tamil Nadu, India.
出版信息
J Indian Soc Pedod Prev Dent. 2008 Mar;26(1):12-7. doi: 10.4103/0970-4388.40315.
BACKGROUND
Oil pulling has been used extensively for many years, without scientific evidence or proof, as a traditional Indian folk remedy to prevent teeth decay, oral malodor, bleeding gums, dryness of throat and cracked lips, and for strengthening the teeth, gums, and jaws.
AIMS
The aim of this study was to evaluate the effect of oil pulling with sesame oil on the count of Streptococcus mutans in plaque and saliva of children, using the Dentocult SM Strip mutans test, and to compare its efficacy with that of chlorhexidine mouthwash.
MATERIALS AND METHODS
Twenty age-matched adolescent boys were selected based on information obtained through a questionnaire. They were divided randomly into two groups: the control or chlorhexidine group (group I) and the study or oil pulling group (group II); there were ten subjects in each group. Plaque and saliva samples were collected from all the 20 subjects on the strips from the Dentocult SM kit and, after incubation, the presence of S. mutans was evaluated using the manufacturers' chart. The study group practiced oil pulling with sesame oil and the control group used chlorhexidine mouthwash for 10 min every day in the morning before brushing. Samples were collected from both groups after 24 h, 48 h, 1 week, and 2 weeks and the efficacy of oil pulling was compared with that of chlorhexidine mouthwash.
RESULTS
There was a reduction in the S. mutans count in the plaque and saliva samples of both the study and the control groups. The reduction in the S. mutans count in the plaque of the study group was statistically significant after 1 and 2 weeks (P=0.01 and P=0.008, respectively); the control group showed significant reduction at all the four time points (P=0.01, P=0.04, P=0.005, and P=0.005, respectively, at 24 h, 48 h, 1 week, and 2 weeks). In the saliva samples, significant reduction in S. mutans count was seen in the control group at 48 h, 1 week, and 2 weeks (P=0.02, P=0.02, P=0.008, respectively).
CONCLUSION
Oil pulling can be used as an effective preventive adjunct in maintaining and improving oral health.
背景
多年来，油拔法作为一种传统的印度民间疗法被广泛使用，但其缺乏科学依据或证据。该疗法用于预防龋齿、口臭、牙龈出血、喉咙干燥和嘴唇干裂，并用于强化牙齿、牙龈和颌骨。
目的
本研究旨在使用Dentocult SM Strip变形链球菌试验评估芝麻油油拔法对儿童牙菌斑和唾液中变形链球菌数量的影响，并将其疗效与洗必泰漱口水进行比较。
材料与方法
通过问卷调查获取信息，选取20名年龄匹配的青春期男孩。他们被随机分为两组：对照组或洗必泰组（I组）和研究组或油拔法组（II组）；每组有10名受试者。从所有20名受试者的Dentocult SM试剂盒试纸条上采集牙菌斑和唾液样本，培养后，使用制造商的图表评估变形链球菌的存在情况。研究组用芝麻油进行油拔法，对照组每天早上刷牙前使用洗必泰漱口水10分钟。在24小时、48小时、1周和2周后从两组采集样本，并将油拔法的疗效与洗必泰漱口水的疗效进行比较。
结果
研究组和对照组的牙菌斑和唾液样本中变形链球菌数量均有所减少。研究组牙菌斑中变形链球菌数量在1周和2周后减少具有统计学意义（分别为P=0.01和P=0.008）；对照组在所有四个时间点均显示出显著减少（在24小时、48小时、1周和2周时分别为P=0.01、P=0.04、P=0.005和P=0.005）。在唾液样本中，对照组在48小时、1周和2周时变形链球菌数量显著减少（分别为P=0.02、P=0.02、P=0.008）。
结论
油拔法可作为维持和改善口腔健康的一种有效预防性辅助方法。
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