Aparicio N J, Casas P R, Galimberti D M, de Laborde N P, Badano A, García E P, Meichi H R, Mirkin A, Szejner M, Jaitt A, Margulies M, Rosner J M
Int J Fertil. 1977;22(1):6-15.
The LH FSH estradiol and progesterone responses to acute stimulation with LH-RH were studied in 12 normal women with ovulatory cycles (4 in the initial follicular phase, 4 in the mid-follicular phase and 4 in the late follicular phase) and in two castrated women, two under hormonal contraception, two with ovarian amenorrhea, twelve with central amenorrhea of no detectable origin (6 with normal and 6 with low basal gonadotrophins), eleven anovulatory patients with pseudomenstruation, two with anorexia nervosa, and two with pituitary amenorrhea. Each woman received a rapid i.v. injection of 100 microgram synthetic LH-RH at 9:00 a.m. Serum levels of LH, FSH, estradiol and progesterone were determined by radioimmunoassay in samples collected before and 60, 120, 240 and 480 minutes after injection. The findings were : 1) A significant rise in estradiol and progesterone levels, in addition to LH and FSH elevation, in normal women; 2) A lack of ovarian steroid response in the castrated women and in ovarian amenorrheas, which suggests that the source of steroid response to stimulation is not extragonadal; 3) Significant differences in the responses of the four hormones to LH-RH in the women with central amenorrhea in comparison with the normal group with great variability of results; the steroid response in the presence of a positive LH response might correlate with the severity and/or prognosis of the disorder, a point deserving further study; 4) In anovulatory women with pseudomenstruation, LH responses for the most part normal, and particularly, progesterone responses.
对12名有排卵周期的正常女性（卵泡期初期4名、卵泡期中期4名、卵泡期晚期4名）、2名去势女性、2名采用激素避孕的女性、2名卵巢性闭经女性、12名原因不明的中枢性闭经女性（基础促性腺激素正常者6名，基础促性腺激素低者6名）、11名有假月经的无排卵患者、2名神经性厌食患者及2名垂体性闭经患者，研究了促黄体生成素释放激素（LH-RH）急性刺激后促黄体生成素（LH）、促卵泡生成素（FSH）、雌二醇和孕酮的反应。每位女性于上午9点静脉快速注射100微克合成LH-RH。通过放射免疫分析法测定注射前及注射后60、120、240和480分钟采集的样本中LH、FSH、雌二醇和孕酮的血清水平。研究结果如下：1）正常女性中，除LH和FSH升高外,雌二醇和孕酮水平也显著升高；2）去势女性和卵巢性闭经患者缺乏卵巢甾体反应，这表明甾体对刺激的反应来源并非性腺外；3）中枢性闭经女性中四种激素对LH-RH的反应与正常组相比存在显著差异，结果变异性很大；LH反应阳性时的甾体反应可能与疾病的严重程度和/或预后相关，这一点值得进一步研究；4）有假月经的无排卵女性，LH反应大多正常，尤其是孕酮反应。
