A frequency-time domain 3D COSY-J technique for measurement of 1H-31P coupling constants in oligonucleotides.

A frequency-time domain 3D NMR technique has been developed for measurement of heteronuclear coupling constants in oligonucleotides employing a combination of COSY and J-resolved techniques. The method employs frequency-selective excitation to generate the omega 1 axis and 2D FT to generate the omega 2 and omega 3 axes. The procedure yields high resolution, especially along the omega 1 axis. The technique is demonstrated on a dinucleotide.