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Sequential backbone assignment in 13C-labeled DNA by the 1H, 13C, 31P triple-resonance experiment, HCP-CCH-COSY.

作者信息

Tate S, Ono A, Kainosho M

机构信息

Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.

出版信息

J Magn Reson B. 1995 Jan;106(1):89-91. doi: 10.1006/jmrb.1995.1016.

DOI:10.1006/jmrb.1995.1016
PMID:7850177
Abstract
摘要

相似文献

1
Sequential backbone assignment in 13C-labeled DNA by the 1H, 13C, 31P triple-resonance experiment, HCP-CCH-COSY.
J Magn Reson B. 1995 Jan;106(1):89-91. doi: 10.1006/jmrb.1995.1016.
2
Sequential correlation of anomeric ribose protons and intervening phosphorus in RNA oligonucleotides by a 1H, 13C, 31P triple resonance experiment: HCP-CCH-TOCSY.通过1H、13C、31P三共振实验（HCP-CCH-TOCSY）对RNA寡核苷酸中异头核糖质子与中间磷的顺序相关性研究
J Biomol NMR. 1995 Jan;5(1):87-92. doi: 10.1007/BF00227473.
3
Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.
J Biomol NMR. 1995 Apr;5(3):315-20. doi: 10.1007/BF00211759.
4
Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.通过二维P(CC)H-TOCSY三重共振核磁共振实验对均匀13C标记的RNA进行序列主链归属。
J Biomol NMR. 1995 Jan;5(1):82-6. doi: 10.1007/BF00227472.
5
Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.均匀13C和15N标记RNA中的顺序分配：HC(N,P)和HC(N,P)-CCH-TOCSY实验
J Biomol NMR. 1996 May;7(3):251-5. doi: 10.1007/BF00202042.
6
The 2D [31P] spin-echo-difference constant-time [13C, 1H]-HMQC experiment for simultaneous determination of 3J(H3'P) and 3J(C4'P) in 13C-labeled nucleic acids and their protein complexes.用于同时测定13C标记核酸及其蛋白质复合物中3J(H3'P)和3J(C4'P)的二维[31P]自旋回波-差分恒时[13C, 1H]-HMQC实验。
J Magn Reson. 1999 Oct;140(2):491-4. doi: 10.1006/jmre.1999.1870.
7
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.一种用于蛋白质1H、13C和15N谱顺序归属的新方法：异核三共振三维核磁共振光谱法。应用于钙调蛋白。
Biochemistry. 1990 May 15;29(19):4659-67. doi: 10.1021/bi00471a022.
8
An efficient procedure for assignment of the proton, carbon and nitrogen resonances in 13C/15N labeled nucleic acids.一种用于确定¹³C/¹⁵N标记核酸中质子、碳和氮共振峰归属的有效方法。
J Mol Biol. 1993 Aug 20;232(4):1141-56. doi: 10.1006/jmbi.1993.1466.
9
Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.采用氢检测二维异核相关光谱法对短DNA双链体的碳-13核磁共振谱进行归属。
Nucleic Acids Res. 1987 Jan 12;15(1):267-75. doi: 10.1093/nar/15.1.267.
10
A frequency-time domain 3D COSY-J technique for measurement of 1H-31P coupling constants in oligonucleotides.
J Biomol NMR. 1991 Jul;1(2):205-8. doi: 10.1007/BF01877231.

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Applications of NMR to structure determination of RNAs large and small.核磁共振在大小RNA结构测定中的应用。
Arch Biochem Biophys. 2017 Aug 15;628:42-56. doi: 10.1016/j.abb.2017.06.003. Epub 2017 Jun 16.
2
Automated NMR resonance assignment strategy for RNA via the phosphodiester backbone based on high-dimensional through-bond APSY experiments.基于高维通过键APSY实验的RNA磷酸二酯主链自动核磁共振共振归属策略。
J Biomol NMR. 2014 Jun;59(2):87-93. doi: 10.1007/s10858-014-9829-z. Epub 2014 Apr 27.
3
A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules.
一种用于关联均匀13C标记的RNA分子中腺嘌呤H2/H8共振的TROSY中继HCCH-COSY实验。
J Biomol NMR. 2001 Jun;20(2):173-6. doi: 10.1023/a:1011214914452.
4
Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids.用于核酸的横向弛豫优化三共振核磁共振实验。
J Biomol NMR. 2000 Apr;16(4):291-302. doi: 10.1023/a:1008388400601.
5
Simple, efficient protocol for enzymatic synthesis of uniformly 13C, 15N-labeled DNA for heteronuclear NMR studies.用于异核核磁共振研究的均匀13C、15N标记DNA酶促合成的简单高效方案。
Nucleic Acids Res. 1998 Jun 1;26(11):2618-24. doi: 10.1093/nar/26.11.2618.
6
Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies.用于核磁共振研究的13C、15N标记DNA的高效酶促合成。
J Biomol NMR. 1997 Oct;10(3):245-53. doi: 10.1023/a:1018358602001.
7
Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.均匀13C和15N标记RNA中的顺序分配：HC(N,P)和HC(N,P)-CCH-TOCSY实验
J Biomol NMR. 1996 May;7(3):251-5. doi: 10.1007/BF00202042.
8
Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.
J Biomol NMR. 1995 Apr;5(3):315-20. doi: 10.1007/BF00211759.