Lutz Rainer, Park Jung, Felszeghy Endre, Wiltfang Jörg, Nkenke Emeke, Schlegel Karl Andreas
Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Erlangen, Germany.
Clin Oral Implants Res. 2008 Jun;19(6):590-9. doi: 10.1111/j.1600-0501.2007.01526.x. Epub 2008 Apr 16.
The aims of this study were to evaluate the rate of bone formation and osseointegration after topical gene delivery with a liposomal vector system carrying bone morphogenetic protein (BMP)-2 cDNA in combination with a collagen carrier and autologous bone as a carrier in freshly created peri-implant bone defects.
Eight domestic pigs received nine calvariae defects each (10 x 7 mm). A dental implant was inserted into the centre of each defect. In the test groups, the remaining space was filled with the liposomal vector/BMP-2 complex combined with a collagen carrier (n=18) or an autologous bone graft (n=18). Control groups were collagen only (n=18) and autologous bone graft only (n=18).
There was a significant difference in mineralisation rate in the BMP-2/bone graft (29.9%+/- 4.8 and 68.3%+/- 7.2) and bone graft only (22.6%+/- 2.6 and 49.4%+/- 13.9) groups after 7 and 28 days. Mineralisation values were also significantly higher in the BMP-2/collagen group (21.2%+/- 16.2 and 53.1%+/- 12.5) compared with the collagen-only group (8.2%+/- 7 and 41%+/- 8.1) in two different regions after 28 days. Also the bone-to-implant contact was significantly increased in the BMP-2/bone graft group after 28 days and in the BMP-2/collagen group after 7 and 28 days compared with their control groups.
The results of this study show a significantly positive effect of liposomal vector/BMP-2 on bone regeneration and osseointegration in bony circumferential peri-implant defects.
本研究旨在评估在新鲜创建的种植体周围骨缺损中，使用携带骨形态发生蛋白（BMP）-2 cDNA的脂质体载体系统与胶原载体及自体骨作为载体进行局部基因递送后，骨形成率和骨整合情况。
八只家猪，每只接受九个颅骨缺损（10×7毫米）。将一枚牙种植体植入每个缺损中心。在试验组中，剩余空间用脂质体载体/BMP-2复合物与胶原载体（n = 18）或自体骨移植（n = 18）填充。对照组分别为仅胶原（n = 18）和仅自体骨移植（n = 18）。
7天和28天后，BMP-2/骨移植组（29.9%±4.8和68.3%±7.2）和仅骨移植组（22.6%±2.6和49.4%±13.9）的矿化率存在显著差异。28天后，在两个不同区域，BMP-2/胶原组（21.2%±16.2和53.1%±12.5）的矿化值也显著高于仅胶原组（8.2%±7和41%±8.1）。同样，28天后BMP-2/骨移植组以及7天和28天后BMP-2/胶原组的骨与种植体接触率与各自对照组相比显著增加。
本研究结果表明脂质体载体/BMP-2对骨周种植体周围骨缺损的骨再生和骨整合具有显著的积极作用。
