Prasongwatana Vitoon, Woottisin Surachet, Sriboonlue Pote, Kukongviriyapan Veerapol
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
J Ethnopharmacol. 2008 May 22;117(3):491-5. doi: 10.1016/j.jep.2008.02.036. Epub 2008 Mar 14.
The Roselle (Hibiscus sabdariffa) was investigated for its uricosuric effect.
A human model with nine subjects with no history of renal stones (non-renal stone, NS) and nine with a history of renal stones (RS) was used in this study. A cup of tea made from 1.5 g of dry Roselle calyces was provided to subjects twice daily (morning and evening) for 15 days. A clotted blood and two consecutive 24-h urine samples were collected from each subject three times: (1) at baseline (control); (2) on days 14 and 15 during the tea drinking period; and (3) 15 days after the tea drinking was stopped (washout). Serum and 24-h urinary samples were analyzed for uric acid and other chemical compositions related to urinary stone risk factors.
All analyzed serum parameters were within normal ranges and similar; between the two groups of subjects and among the three periods. Vis-à-vis the urinary parameters, most of the baseline values for both groups were similar. After taking the tea, the trend was an increase in oxalate and citrate in both groups and uric acid excretion and clearance in the NS group. In the RS group, both uric acid excretion and clearance were significantly increased (p<0.01). When the fractional excretion of uric acid (FEUa) was calculated, the values were clearly increased in both the NS and SF groups after the intake of tea and returned to baseline values in the washout period. These changes were more clearly observed when the data for each subject was presented individually.
Our data demonstrate a uricosuric effect of Roselle calyces. Since the various chemical constituents in Roselle calyces have been identified, the one(s) exerting this uricosuric effect need to be identified.
研究玫瑰茄（Hibiscus sabdariffa）的促尿酸排泄作用。
本研究采用了一个人体模型，其中包括9名无肾结石病史的受试者（非肾结石组，NS）和9名有肾结石病史的受试者（肾结石组，RS）。每天为受试者提供两杯由1.5克干玫瑰茄花萼制成的茶（早晚各一杯），持续15天。从每位受试者身上采集三次凝血血液和两份连续的24小时尿液样本：（1）基线期（对照）；（2）饮茶期的第14天和第15天；（3）停止饮茶15天后（洗脱期）。分析血清和24小时尿液样本中的尿酸以及与尿路结石风险因素相关的其他化学成分。
所有分析的血清参数均在正常范围内且相似；在两组受试者之间以及三个时期之间。关于尿液参数，两组的大多数基线值相似。饮茶后，两组的草酸盐和柠檬酸盐均呈上升趋势，非肾结石组的尿酸排泄和清除率也有所增加。在肾结石组中，尿酸排泄和清除率均显著增加（p<0.01）。计算尿酸分数排泄（FEUa）时，饮茶后非肾结石组和肾结石组的值均明显升高，并在洗脱期恢复到基线值。当单独呈现每位受试者的数据时，这些变化更为明显。
我们的数据证明了玫瑰茄花萼具有促尿酸排泄作用。由于玫瑰茄花萼中的各种化学成分已被鉴定出来，因此需要确定发挥这种促尿酸排泄作用的成分。
