Keating S T, Elkinton J S, Burand J P, Podgwaite J D, Ferguson C S
Department of Entomology, University of Massachusetts, Amherst 01003.
J Econ Entomol. 1991 Aug;84(4):1329-33. doi: 10.1093/jee/84.4.1329.
DNA hybridization assays were used to detect the presence of viral DNA in gypsy moth (Lymantria dispar L.) larvae collected weekly from high density populations or reared from field-collected egg masses. DNA was extracted from larvae, bound to nitrocellulose filters, and hybridized with digoxigenin-labeled L. dispar NPV (LdNPV) DNA probes. The virus incidence determined from DNA hybridization assays was compared with that determined with conventional microscopic examination of larvae for polyhedral inclusion bodies. Among neonates reared from field-collected egg masses, average mortality from LdNPV (15.4%) within 10 d after hatch was not significantly different from the percentage of extracts containing LdNPV DNA (14.8%) found among larvae frozen 5 d after hatch before any mortality occurred. Field-collected larvae were split into two groups: half were frozen immediately and probed for LdNPV DNA and the other half were reared on artificial diet. The proportion containing LdNPV DNA closely approximated the proportion that died within 6 d of collection, but the proportion that died within 13 d of collection was underestimated.
采用DNA杂交试验检测每周从高密度种群采集或由野外采集的卵块饲养的舞毒蛾（Lymantria dispar L.）幼虫中病毒DNA的存在情况。从幼虫中提取DNA，将其结合到硝酸纤维素滤膜上，并用地高辛标记的舞毒蛾核型多角体病毒（LdNPV）DNA探针进行杂交。将DNA杂交试验确定的病毒发生率与通过对幼虫进行常规显微镜检查多面体包涵体确定的病毒发生率进行比较。在由野外采集的卵块饲养的新生幼虫中，孵化后10天内LdNPV的平均死亡率（15.4%）与孵化后5天在未出现任何死亡之前冷冻的幼虫中含有LdNPV DNA的提取物百分比（14.8%）无显著差异。将野外采集的幼虫分成两组：一半立即冷冻并检测LdNPV DNA，另一半用人工饲料饲养。含有LdNPV DNA的比例与采集后6天内死亡的比例非常接近，但采集后13天内死亡的比例被低估了。
