在液体或固体培养基中生长。
Growth in liquid or solid media.
作者信息
Elbing K L, Brent R
机构信息
Clark and Elbing LLP, Boston, Massachusetts, USA.
出版信息
Curr Protoc Protein Sci. 2001 May;Appendix 4:Appendix 4B. doi: 10.1002/0471140864.psa04bs13.
PMID:18429077
Abstract
This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.
摘要
本附录介绍了培养过夜（及更大规模）培养物、监测生长情况以及通过连续稀释对细菌培养物进行滴定和分离的基本程序。此外，还提供了通过划线和涂布平板分离单菌落的方案。同时也详细介绍了影印接种以及菌株保存/复苏的方法。
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