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波兰西尼罗河病毒潜在脊椎动物宿主的血清学调查。

Serologic survey of potential vertebrate hosts for West Nile virus in Poland.

作者信息

Hubálek Zdenek, Wegner Elzbieta, Halouzka Jirí, Tryjanowski Piotr, Jerzak Leszek, Sikutová Silvie, Rudolf Ivo, Kruszewicz Andrzej G, Jaworski Zbigniew, Wlodarczyk Radoslaw

机构信息

Institute of Vertebrate Biology, Academy of Sciences, Brno, Czech Republic.

出版信息

Viral Immunol. 2008 Jun;21(2):247-53. doi: 10.1089/vim.2007.0111.

DOI:10.1089/vim.2007.0111
PMID:18433332
Abstract

A survey for antibodies to West Nile virus (WNV; genus ,Flavivirus) was carried out by plaque-re-duction neutralization microtesting in 78 horses, 20 domestic chickens, and 97 wild birds belonging to 10 species from different areas in Poland. Specific antibodies were detected in five juvenile (hatching-year) birds collected in 2006: three white storks (Ciconia ciconia) in a wildlife rehabilitation center (5.4% of all examined storks; the antibody titers in each bird were 1:320, 1:160, and 1:20), one free-living mute swan (Cygnus olor; the titer was 1:20), and one hooded crow (Corvus corone cornix; the titer 1:20) in a wildlife rehabilitation center; thus the overall seropositivity to WNV was 5.2% among all the birds sampled. These data do not rule out the presence of WNV activity in Poland with 100% certainty, but they indicate a significant trace that demands verification. In addition, one black-headed gull (Larus ridibundus) had neutralizing antibodies for the Usutu Flavivirus, the first case recorded in Poland.

摘要

通过蚀斑减少中和微量试验,对来自波兰不同地区的78匹马、20只家鸡和属于10个物种的97只野生鸟类进行了西尼罗河病毒(WNV;黄病毒属)抗体检测。在2006年采集的5只幼鸟(当年孵化)中检测到特异性抗体:野生动物康复中心的3只白鹳(白鹳)(占所有检测鹳的5.4%;每只鸟的抗体滴度分别为1:320、1:160和1:20)、1只自由生活的疣鼻天鹅(疣鼻天鹅;滴度为1:20)和野生动物康复中心的1只秃鼻乌鸦(小嘴乌鸦;滴度为1:20);因此,在所有采样鸟类中,对WNV的总体血清阳性率为5.2%。这些数据不能100%确定地排除波兰存在WNV活动的可能性,但它们表明了一个需要核实的重要线索。此外,1只黑头鸥(红嘴鸥)具有针对乌苏图黄病毒的中和抗体,这是波兰记录的首例。

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