Komoto Satoshi, Kugita Masanori, Sasaki Jun, Taniguchi Koki
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
J Virol. 2008 Jul;82(13):6753-7. doi: 10.1128/JVI.00601-08. Epub 2008 Apr 23.
Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.
利用最近开发的轮状病毒反向遗传学技术,构建了具有cDNA衍生嵌合VP4的重组轮状病毒(RV)。拯救出的病毒KU//rVP4(SA11)-II(DS-1)含有基于SA11(猿猴RV毒株,G3P[2])的VP4,其中VP5*上的一个交叉反应性中和表位(氨基酸381至401)被不同P型DS-1(人RV毒株,G2P[4])的相应序列所取代。用一组抗VP4和抗VP7中和单克隆抗体进行的血清学分析表明,拯救出的病毒在其VP4表面带有交叉反应性中和表位的新型抗原嵌合体。这是关于产生具有人工氨基酸取代的重组RV的首次报道。