[酶制剂中的霉菌毒素。第二部分。柄曲霉素的检测和测定方法]
[Mycotoxins in enzyme preparations. Part II. Method of detection and determination of sterigmatocystin].
作者信息
Czerwiecki L
机构信息
Zakładu Analizy Zywności Instytutu Biotechnologii Przemysłu Rolno-Spozywczego.
出版信息
Rocz Panstw Zakl Hig. 1991;42(4):393-9.
PMID:1844014
Abstract
A simple method for the detection and semiquantitative determination of sterigmatocystin in enzyme preparations was described. The method comprises extraction with acetonitrile and a 4% aqueous KCL solution, column chromatographic purification of the crude extracts on silica gel, and two-dimensional thin-layer chromatography. The mean recovery by this method is from 71 to 79%, and the detectability of sterigmatocystin is 20 micrograms/kg.
摘要
描述了一种用于检测和半定量测定酶制剂中柄曲霉素的简单方法。该方法包括用乙腈和4%的氯化钾水溶液萃取，在硅胶上对粗提物进行柱色谱纯化，以及二维薄层色谱法。该方法的平均回收率为71%至79%，柄曲霉素的检测限为20微克/千克。
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