[酶制剂中的霉菌毒素。III. 玉米赤霉烯酮的检测和测定方法]
[Mycotoxins in enzyme preparations. III. Method of detection and determination of zearalenone].
作者信息
Czerwiecki L
机构信息
Zakładu Analizy Zywności Instytutu Biotechnologii Przemysłu Rolno-Spozywczego.
出版信息
Rocz Panstw Zakl Hig. 1993;44(2-3):147-50.
PMID:8016535
Abstract
A simple method for the detection and semiquantitative determination of zearalenone in enzyme preparations was described. The method comprises extraction with chloroform and acetone (80 + 20), mini-column chromatographic purification of the crude extracts on silicagel, and two-dimensional thin-layer chromatography. The mean recovery by this method in from 74 to 80% and detectability of zearalenone is 50 micrograms/kg.
摘要
描述了一种检测和半定量测定酶制剂中玉米赤霉烯酮的简单方法。该方法包括用氯仿和丙酮（80+20）萃取，在硅胶上对粗提物进行微型柱色谱纯化，以及二维薄层色谱法。该方法的平均回收率为74%至80%，玉米赤霉烯酮的检测限为50微克/千克。
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