Koko W S, Mesaik M Ahmed, Yousaf S, Galal M, Choudhary M Iqbal
Medicinal and Aromatic Plants Research Institute, National Center for Research, Khartoum, Sudan.
J Ethnopharmacol. 2008 Jun 19;118(1):26-34. doi: 10.1016/j.jep.2008.03.007. Epub 2008 Mar 18.
Ethanolic extracts of 23 medicinal plants, commonly used in Sudanese folk medicines against infectious diseases, were investigated for their immunomodulating activity using luminol/lucigenin-based chemiluminescence assay. Preliminary screenings on whole blood oxidative burst activity showed inhibitory activities of 14 plant extracts, while only one plant, Balanites aegyptiaca fruits exhibited a proinflammatory activity. Further investigation was conducted by monitoring their effects on oxidative burst of isolated polymorphonuclear cells (PMNs) and mononuclear cells (MNCs) by using two different phagocytosis activators (serum opsonizing zymosan-A and PMA). Results obtained showed that the fruits and barks of Acacia nilotica, and leaves and barks of Khaya senegalensis, possess average inhibitory effects in the range of 70.7, 67.1, 69.5 and 67.4% on both types of phagocytes (PMNs and MNCs), respectively, at a 6.25 microg/mL concentration. Moderate inhibitory activity (52.2%) was exerted by the aerial parts of Xanthium brasilicum, while the rest of the plants showed only a weak inhibitory activity. The inhibition of oxidative burst activity was found to be irreversible in most of the extracts, except for Peganum harmala, Tephrosia apollinea, Tinospora bakis, and Vernonia amygdalina. Interestingly, the fruits of Balanites aegyptiaca exhibited a moderate proinflammatory effect (37-40.4% increases in ROS level compared to the control) at 25-100 microg/mL concentration in the case of whole blood along with PMNs phagocyte activity. The Tinospora bakis extract showed proinflammatory response at a low concentration (6.25 microg/mL) during activation with PMA. None of these extracts affected PMNs viability (90-98%) upon 2 h incubation, except of the ethanolic extracts of Acacia nilotica fruits and Balanites aegyptiaca barks.
对苏丹民间用于治疗传染病的23种药用植物的乙醇提取物，采用基于鲁米诺/光泽精的化学发光分析法研究其免疫调节活性。对全血氧化爆发活性的初步筛选显示，14种植物提取物具有抑制活性，而只有一种植物——埃及 balanites 果实表现出促炎活性。通过使用两种不同的吞噬作用激活剂（血清调理酵母聚糖 -A 和佛波酯）监测它们对分离的多形核细胞（PMN）和单核细胞（MNC）氧化爆发的影响，进行了进一步研究。结果表明，在浓度为6.25微克/毫升时，阿拉伯胶树的果实和树皮以及塞内加尔桃花心木的叶子和树皮，对两种类型的吞噬细胞（PMN和MNC）分别具有平均70.7%、67.1%、69.5%和67.4%的抑制作用。巴西苍耳地上部分具有中等抑制活性（52.2%），而其余植物仅表现出微弱的抑制活性。除了骆驼蓬、灰叶、锡兰藤和斑鸠菊外，大多数提取物对氧化爆发活性的抑制作用是不可逆的。有趣的是，在全血以及PMN吞噬细胞活性的情况下，埃及 balanites 果实在25 - 100微克/毫升浓度下表现出中等促炎作用（与对照相比，ROS水平增加37 - 40.4%）。锡兰藤提取物在用佛波酯激活时，在低浓度（6.25微克/毫升）下表现出促炎反应。除了阿拉伯胶树果实和埃及 balanites 树皮的乙醇提取物外，这些提取物在孵育2小时后均不影响PMN的活力（90 - 98%）。
