Kondakova Anna N, Ho Nathan, Bystrova Olga V, Shashkov Alexander S, Lindner Buko, Creuzenet Carole, Knirel Yuriy A
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Carbohydr Res. 2008 Jun 9;343(8):1383-9. doi: 10.1016/j.carres.2008.04.003. Epub 2008 Apr 7.
The full structure of the long- and short-chain O-antigen of Yersinia pseudotuberculosis O:2a containing two uncommon deoxy sugars, abequose and 6-deoxy-d-manno-heptose (6dmanHep), was established, for the first time, by sugar analysis, NMR spectroscopy, and high-resolution ESIMS. Similar structural studies were also performed on two O:2a mutants with single disruption of 6dmanHep synthesis pathway genes each, which synthesize modified long-chain (dmhA mutant) and short-chain (both dmhA and dmhB mutants) O-antigens with 6dmanHep replaced by its putative biosynthetic precursor, D-glycero-D-manno-heptose.
首次通过糖分析、核磁共振光谱和高分辨率电喷雾离子质谱法确定了假结核耶尔森氏菌O:2a的长链和短链O抗原的完整结构，该抗原含有两种不常见的脱氧糖，阿比可糖和6-脱氧-D-甘露庚糖（6dmanHep）。还对两个O:2a突变体进行了类似的结构研究，每个突变体分别单破坏6dmanHep合成途径基因，它们合成修饰的长链（dmhA突变体）和短链（dmhA和dmhB突变体）O抗原，其中6dmanHep被其假定的生物合成前体D-甘油-D-甘露庚糖取代。
