循环纤维细胞在增殖性玻璃体视网膜病变视网膜前膜的肌成纤维细胞群体中发挥作用。

Circulating fibrocytes contribute to the myofibroblast population in proliferative vitreoretinopathy epiretinal membranes.

作者信息

Abu El-Asrar A M, Struyf S, Van Damme J, Geboes K

机构信息

Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

出版信息

Br J Ophthalmol. 2008 May;92(5):699-704. doi: 10.1136/bjo.2007.134346.

DOI:10.1136/bjo.2007.134346

PMID:18441176

Abstract

BACKGROUND/AIMS: Fibrocytes, circulating cells that co-express markers of haematopoietic stem cells, leucocytes and fibroblast products, traffic to sites of tissue injury, differentiate into myofibroblasts and contribute to wound healing and fibrosis. We investigated the presence of fibrocytes and the expression of their chemotactic pathways CCL21/CCR7 and CXCL12/CXCR4 in proliferative vitreoretinopathy (PVR) epiretinal membranes.

METHODS

Sixteen membranes were studied by immunohistochemical techniques.

RESULTS

Cells expressing alpha-smooth-muscle actin (alpha-SMA), a marker of differentiation of fibrocytes into myofibroblasts, were present in all membranes. Cells expressing the haematopoietic stem-cell antigen CD34, the leucocyte common antigen CD45, CCR7, CXCR4, CCL21 and CXCL12 were noted in 50%, 75%, 68.8%, 100%, 80% and 93.8% of the membranes, respectively. Double immunohistochemistry indicated that all cells expressing CD34, CD45, CCR7, CXCR4, CCL21 and CXCL12 co-expressed alpha-SMA. The number of cells expressing CD34 correlated significantly with the numbers of cells expressing CXCL12 (r(s) = 0.567; p = 0.022) and CCL21 (r(s) = 0.534; p = 0.04).

CONCLUSIONS

Circulating fibrocytes may function as precursors of myofibroblasts in PVR membranes.

摘要

背景/目的：纤维细胞是一种循环细胞，共表达造血干细胞、白细胞和成纤维细胞产物的标志物，可迁移至组织损伤部位，分化为肌成纤维细胞，并参与伤口愈合和纤维化过程。我们研究了增殖性玻璃体视网膜病变（PVR）视网膜前膜中纤维细胞的存在情况及其趋化途径CCL21/CCR7和CXCL12/CXCR4的表达。

方法

采用免疫组织化学技术研究16个视网膜前膜。

结果

所有视网膜前膜中均存在表达α-平滑肌肌动蛋白（α-SMA）的细胞，α-SMA是纤维细胞分化为肌成纤维细胞的标志物。分别在50%、75%、68.8%、100%、80%和93.8%的视网膜前膜中发现了表达造血干细胞抗原CD34、白细胞共同抗原CD45、CCR7、CXCR4、CCL21和CXCL12的细胞。双重免疫组织化学表明，所有表达CD34、CD45、CCR7、CXCR4、CCL21和CXCL12的细胞均共表达α-SMA。表达CD34的细胞数量与表达CXCL12的细胞数量（r(s)=0.567；p=0.022）和CCL21的细胞数量（r(s)=0.534；p=0.04）显著相关。