Research Laboratories in Ophthalmology, IRCCS-Fondazione Bietti, Rome, Italy.
Retinal Unit, IRCCS-Fondazione Bietti, Rome, Italy.
Biomed Res Int. 2022 Dec 16;2022:7497816. doi: 10.1155/2022/7497816. eCollection 2022.
A plethora of inflammatory, angiogenic, and tissue remodeling factors has been reported in idiopathic epiretinal membranes (ERMs). Herein we focused on the expression of a few mediators (oxidative, inflammatory, and angiogenic/vascular factors) by means of short-term vitreal cell cultures and biomolecular analysis.
Thirty-nine (39) ERMs and vitreal samples were collected at the time of vitreoretinal surgery and biomolecular analyses were performed in clear vitreous, vitreal cell pellets, and ERMs. ROS products and iNOS were investigated in adherent vitreal cells and/or ERMs, and iNOS, VEGF, Ang-2, IFN, IL18, and IL22 were quantified in vitreous (ELISA/Ella, IF/WB); transcripts specific for , , , , and were detected in ERMs (PCR). Biomolecular changes were analyzed and correlated with disease severity.
The higher ROS production was observed in vitreal cells at stage 4, and iNOS was found in ERMs and increased in the vitreous as early as at stage 3. Both and were upregulated at all stages, while was increased at stage 3. and were positively and inversely related with . While transcripts were always upregulated, was upregulated at stage 3 and inverted at stage 4. No significant changes occurred in the release of angiogenic (VEGF, Ang-2) and proinflammatory (IL18, IL22 and IFN) mediators between all stages investigated.
ROS production was strictly associated with and overexpression and increased depending on ERM stadiation. The higher expression occurred as early as stage 3, with respect to and . These last mediators might have potential prognostic values in ERMs as representative of an underneath retinal damage.
特发性视网膜前膜（ERM）中有大量的炎症、血管生成和组织重塑因子被报道。在此，我们通过短期玻璃体细胞培养和生物分子分析，重点研究了一些介质（氧化、炎症和血管生成/血管因子）的表达。
在玻璃体视网膜手术时收集了 39 例（39 例）ERM 和玻璃体样本，并在透明玻璃体、玻璃体细胞沉淀和 ERM 中进行了生物分子分析。在贴壁玻璃体细胞和/或 ERM 中研究了 ROS 产物和 iNOS，在玻璃体中（ELISA/Ella、IF/WB）定量了 iNOS、VEGF、Ang-2、IFN、IL18 和 IL22；在 ERM 中检测了 、 、 、 和 的特异性转录本（PCR）。分析了生物分子变化，并与疾病严重程度相关联。
在第 4 期，观察到玻璃体细胞中更高的 ROS 产生，而 iNOS 存在于 ERM 中，并在第 3 期就增加到玻璃体中。 和 均在所有阶段上调，而 则在第 3 期上调。 和 与 呈正相关和负相关。 转录本始终上调，而 在第 3 期上调，在第 4 期反转。在研究的所有阶段，血管生成（VEGF、Ang-2）和促炎（IL18、IL22 和 IFN）介质的释放没有明显变化。
ROS 产物与 和 过表达密切相关，并随着 ERM 分期的增加而增加。较高的 表达发生在第 3 期，与 和 相比。这些最后一种介质可能具有 ERM 的潜在预后价值，因为它们代表了视网膜下的损伤。
