水痘带状疱疹病毒开放阅读框58基因在细胞培养中对病毒复制是非必需的。
Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture.
作者信息
Yoshii Hironori, Sadaoka Kay, Matsuura Masaaki, Nagaike Kazuhiro, Takahashi Michiaki, Yamanishi Koichi, Mori Yasuko
机构信息
Laboratory of Virology and Vaccinology, Division of Biomedical Research, National Institute of Biomedical Innovation, Osaka, Japan.
出版信息
Virol J. 2008 Apr 30;5:54. doi: 10.1186/1743-422X-5-54.
BACKGROUND
Open reading frame 58 (ORF58) of varicella-zoster virus (VZV) lies at the 3'end of the Unique long (UL) region and its functional is unknown. In order to clarify whether ORF58 is essential for the growth of VZV, we constructed a deletion mutant of ORF58 (pOka-BACDelta58) from the Oka parental genome cloned into a bacterial artificial chromosome (pOka-BAC).
RESULTS
The ORF58-deleted virus (rpOkaDelta58) was reconstituted from the pOka-BACDelta58 genome in MRC-5 cells, indicating that the ORF58 gene is non-essential for virus growth. Comparison of the growth rate of rpOkaDelta58 and recombinant wild-type virus by assessing plaque sizes revealed no significant differences between them both in MRC-5 cells and malignant melanoma cells.
CONCLUSION
This study shows that the ORF58 gene is dispensable for viral replication and does not affect the virus' ability to form plaques in vitro.
背景
水痘 - 带状疱疹病毒（VZV）的开放阅读框58（ORF58）位于独特长区域（UL）的3'端，其功能尚不清楚。为了阐明ORF58对VZV生长是否必不可少，我们从克隆到细菌人工染色体（pOka - BAC）中的Oka亲本基因组构建了ORF58缺失突变体（pOka - BACDelta58）。
结果
从pOka - BACDelta58基因组在MRC - 5细胞中重建了ORF58缺失病毒（rpOkaDelta58），表明ORF58基因对病毒生长不是必需的。通过评估噬斑大小比较rpOkaDelta58和重组野生型病毒的生长速率，发现在MRC - 5细胞和恶性黑色素瘤细胞中它们之间没有显著差异。
结论
本研究表明ORF58基因对于病毒复制是可有可无的，并且不影响病毒在体外形成噬斑的能力。
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