Gao Jiayu, Sanchez-Medina Alberto, Pendry Barbara A, Hughes Michael J, Webb Geoffrey P, Corcoran Olivia
Medicines Research Group, School of Health and Bioscience, University of East London, Stratford, London UK.
J Pharm Pharm Sci. 2008;11(1):77-87. doi: 10.18433/j39g6v.
To compare the flavonoid biomarker content (baicalin, baicalein and wogonin) of eleven commercial tinctures derived from Scutellaria lateriflora aerial parts (n=7) and Scutellaria baicalensis root (n=4). S. lateriflora tinctures are used in by western herbal practitioners to treat anxiety whereas S. baicalensis tinctures are used to treat inflammatory disease.
Baicalin and baicalein were purchased from Aldrich Chemical Co. and Wogonin was purchased from ChromaDex. The internal standard (4-hydroxybenzoic acid) was obtained from Acros Organics. The column used was a Luna C18, 5 m (150 x 4.6 mm, Phenomenex) maintained at ambient room temperature. A HP1050 HPLC system was used, comprising a gradient pump with degasser, a variable wavelength UV detector set to 270 nm, and an autosampler. Gradient elution was performed using 0.1% formic acid (eluent A) and methanol (eluent B). The gradient elution initial conditions were 45% B with linear gradient to 60% from 2 to 10 min, followed by linear gradient to 70% B at 30 min, and then linear gradient to 99% B at 31 min, this proportion being maintained for 1 min. The mobile phase was then returned to initial conditions at 33 min and maintained until the end of the run at 35 min. The flow rate was 1 mL/min. The assay was validated for sensitivity, accuracy and reproducibility.
The concentration range of biomarkers (baicalin, baicalein and wogonin) in commercial tinctures is reported for S. lateriflora (baicalin: 0-12.66 mg/mL; baicalein: 0-0.63 mg/mL; wogonin: 0-0.16 mg/mL) and for S. baicalensis (baicalin: 0.12-10.61 mg/mL; baicalein: 0.52-5.88 mg/mL; wogonin: 0.08-1.61 mg/mL).
The wide variability in biomarker concentrations between commercial tinctures has important implications for the manufacturers of commercial tinctures, for herbal practitioners in the choice of tinctures and not least for pharmacology and clinical researchers.
比较11种市售酊剂中黄酮类生物标志物（黄芩苷、黄芩素和汉黄芩素）的含量，这些酊剂分别来源于半枝莲地上部分（n = 7）和黄芩根（n = 4）。西方草药从业者使用半枝莲酊剂治疗焦虑症，而黄芩酊剂则用于治疗炎症性疾病。
黄芩苷和黄芩素购自奥德里奇化学公司，汉黄芩素购自ChromaDex公司。内标（4 - 羟基苯甲酸）购自爱克罗斯有机化学品公司。使用的色谱柱为Luna C18，5μm（150×4.6 mm，菲罗门公司），柱温保持在室温。采用HP1050高效液相色谱系统，包括带脱气机的梯度泵、设定为270 nm的可变波长紫外检测器和自动进样器。使用0.1%甲酸（洗脱液A）和甲醇（洗脱液B）进行梯度洗脱。梯度洗脱初始条件为45% B，在2至10分钟内线性梯度至60% B，然后在30分钟时线性梯度至70% B，接着在31分钟时线性梯度至99% B，并保持此比例1分钟。然后在33分钟时流动相回到初始条件并保持至35分钟运行结束。流速为1 mL/min。对该分析方法的灵敏度、准确度和重现性进行了验证。
报告了市售酊剂中半枝莲（黄芩苷：0 - 12.66 mg/mL；黄芩素：0 - 0.63 mg/mL；汉黄芩素：0 - 0.16 mg/mL）和黄芩（黄芩苷：0.12 - 10.61 mg/mL；黄芩素：0.52 - 5.88 mg/mL；汉黄芩素：0.08 - 1.61 mg/mL）生物标志物的浓度范围。
市售酊剂中生物标志物浓度的广泛差异对酊剂制造商、草药从业者在酊剂选择方面具有重要意义，对药理学和临床研究人员也尤为重要。
