College of Chemistry, Jilin University, Qianjin Street, Changchun 130012, China.
J Chromatogr A. 2010 Mar 19;1217(12):1875-81. doi: 10.1016/j.chroma.2010.01.050. Epub 2010 Jan 25.
An on-line method, based on coupling dynamic ultrasonic extraction (DUE), continuously sampling the suspension of sample and solvent, high performance liquid chromatographic separation with diode array detection, has been developed for the determination of the flavonoids, including baicalin, baicalein and wogonin, from the root of Scutellaria baicalensis Georgi. Variables influencing the DUE were evaluated by orthogonal test. The extraction yields of baicalin, baicalein and wogonin in the roots of S. baicalensis Georgi obtained from five different cultivated areas are 73.8-131.5 microg mg(-1) (RSD< or =6.24%), 6.8-15.9 microg mg(-1) (RSD< or =5.36%) and 4.4-14.3 microg mg(-1) (RSD< or =5.30%), respectively. The limits of detection for baicalin, baicalein and wogonin are 0.30, 0.37 and 0.41 microg mL(-1), respectively. Linearity is from 0.55 to 109 microg mL(-1) for baicalin, from 0.51 to 105 microg mL(-1) for baicalein and from 0.53 to 102 microg mL(-1) for wogonin. Compared with off-line continuous flow-DUE, the proposed method would be more convenient for the determination of the analytes and the rapid optimization of the extraction process. The extraction yields of flavonoids obtained by the proposed method are comparable with those obtained by dynamic microwave assisted extraction, static ultrasonic extraction and reflux extraction. The result indicated that the proposed method is suitable to determine the active components in Chinese herbal medicine.
一种基于在线动态超声提取（DUE）的方法，连续采集样品和溶剂的悬浮液，高效液相色谱分离与二极管阵列检测，已被开发用于测定黄芩根中的黄酮类化合物，包括黄芩苷、黄芩素和汉黄芩素。通过正交试验评价了影响 DUE 的变量。从五个不同种植区获得的黄芩根中的黄芩苷、黄芩素和汉黄芩素的提取率分别为 73.8-131.5μg mg（-1）（RSD<或=6.24%）、6.8-15.9μg mg（-1）（RSD<或=5.36%）和 4.4-14.3μg mg（-1）（RSD<或=5.30%）。黄芩苷、黄芩素和汉黄芩素的检出限分别为 0.30、0.37 和 0.41μg mL（-1）。线性范围为 0.55-109μg mL（-1）的黄芩苷、0.51-105μg mL（-1）的黄芩素和 0.53-102μg mL（-1）的汉黄芩素。与离线连续流动-DUE 相比，该方法更便于测定分析物和快速优化提取过程。该方法得到的黄酮类化合物提取率与动态微波辅助提取、静态超声提取和回流提取相当。结果表明，该方法适用于测定中草药中的活性成分。
