Yang Ting, Yang Yi-Hua, Yang Ju-Yun, Chen Ben-Mei, Duan Ju-Ping, Yu Shu-Yi, Ouyang Hong-Tao, Cheng Jun-Ping, Chen Yu-Xiang
School of Biosciences and Technologies, Central South University, Changsha, Hunan 410013, People's Republic of China.
Phytochem Anal. 2008 Nov-Dec;19(6):487-92. doi: 10.1002/pca.1071.
A HPLC-MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil-Keystone Hypurity C(18) column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC-MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC-MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H. diffusa was well distinguished from Hedyotis chrysotricha by HPLC-MS. Therefore the establishment of fingerprint of H. diffusa is critical in assessing and controlling its overall quality.
基于10批白花蛇舌草中主要化学成分一致的色谱特征，建立了一种高效液相色谱-质谱联用指纹图谱方法。采用甲醇：水：乙酸作为流动相，在Hypersil-Keystone Hypurity C(18)柱上进行色谱分离。通过高效液相色谱-质谱联用分析了包括齐墩果酸、熊果酸和阿魏酸在内的主要化合物。通过与标准化合物的数据进行比较来确定它们的分析结果。高效液相色谱-质谱联用指纹图谱成功应用于分析和区分来自不同地理来源或加工方法的样品。通过高效液相色谱-质谱联用，白花蛇舌草与黄毛耳草得到了很好的区分。因此，建立白花蛇舌草指纹图谱对于评估和控制其整体质量至关重要。
