高效液相色谱法测定黄毛耳草中甲基异茜草素-3-甲酯的含量
[Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC].
作者信息
Li Jing, Zhou Guoping, Li Caitang, Wen Ping, Ying Ping, Yu Jinbao, Wu Huodi
机构信息
Jiangxi Provincial Institution of Traditional Chinese Medicine, Nanchang, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2619-21.
OBJECTIVE
To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.
METHOD
The determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.
RESULT
There is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.
CONCLUSION
This method is convenient, quick, and is applicable to the quality control of H. chrysotricha.
目的
建立一种测定黄毛耳草中甲基异斯堪德尔苷的高效液相色谱法。
方法
采用HC-C18柱，以乙腈-水（7:93）为流动相进行测定，检测波长设定为238nm。
结果
甲基异斯堪德尔苷在22.08 - 552mg L(-1)范围内线性关系良好，平均回收率为97.7%（n = 6），相对标准偏差为0.72%。
结论
该方法简便、快速，适用于黄毛耳草的质量控制。
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