Li Jing, Zhou Guoping, Li Caitang, Wen Ping, Ying Ping, Yu Jinbao, Wu Huodi
Jiangxi Provincial Institution of Traditional Chinese Medicine, Nanchang, China.
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2619-21.
To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.
The determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.
There is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.
This method is convenient, quick, and is applicable to the quality control of H. chrysotricha.
建立一种测定黄毛耳草中甲基异斯堪德尔苷的高效液相色谱法。
采用HC-C18柱，以乙腈-水（7:93）为流动相进行测定，检测波长设定为238nm。
甲基异斯堪德尔苷在22.08 - 552mg L(-1)范围内线性关系良好，平均回收率为97.7%（n = 6），相对标准偏差为0.72%。
该方法简便、快速，适用于黄毛耳草的质量控制。
