Xing Zhan-Fen, Cheng Hong-Da, Zhang Ping-Ping
Zhong Yao Cai. 2014 May;37(5):789-91.
To establish HPLC fingerprint of Hedyotis chrysotricha.
Hypersil BDS C18 (250 mm x 4.6 mm, 5 microm) column was used with methanol and 0.1% acetic acid solution as mobile phase in gradient mode, the flow rate was 1.0 mL/ min. The detection wavelength was 345 nm and the temperature of the column was set at 30 degrees C. Using ursolic acid as the reference standard, the mutual mode of Hedyotis chrysotricha containing nine common peaks was set up. The similarity of Hedyotis chrysotricha from different habitats was analyzed.
The fingerprints of Hedyotis chrysotricha from different habitats were similar, but also had habitat characteristics.
The HPLC fingerprint can be used for identification and quality control of Hedyotis chrysotricha.
建立黄毛耳草的高效液相色谱指纹图谱。
采用Hypersil BDS C18（250mm×4.6mm，5μm）色谱柱，以甲醇和0.1%醋酸溶液为流动相进行梯度洗脱，流速为1.0mL/min。检测波长为345nm，柱温设定为30℃。以熊果酸为对照品，建立了含有9个共有峰的黄毛耳草的共有模式。分析了不同产地黄毛耳草的相似度。
不同产地黄毛耳草的指纹图谱既有相似性，又具有产地特征。
该高效液相色谱指纹图谱可用于黄毛耳草的鉴别和质量控制。
