源自人类白血病的连续细胞培养物释放的细胞外颗粒的表征

Characterization of extracellular particles released from continuous cell cultures derived from human leukemia.

作者信息

Smith C C, Maverakis N H, Ackermann W W

出版信息

Proc Soc Exp Biol Med. 1976 Sep;152(4):645-50. doi: 10.3181/00379727-152-39459.

Abstract

Extracellular particles, with a density of 1.18-1.22 g/cm3 in sucrose, were detected in the culture medium of a continuous cell line (JIII) derived from a patient with monocytic leukemia. These particles contained RNA, DNA, and a DNA polymerase. They synthesized DNA with endogenous templates and primers and also used exogenous DNA but not poly(rC) oligo(dG) as a template. Pretreatment with Nonidet P-40 stimulated DNA polymerase activity while treatment with ribonuclease partially inhibited the enzyme activity. Fluorescent antibodies made to the particles stained both JIII and Z-597 cells derived from human leukemias but not other types of human or nonhuman cultured cells tested. The particles do not appear to be oncornaviruses but may be a particulate antigen associated with malignant cells of hemopoietic and lymphoid origin.

摘要

在源自一名单核细胞白血病患者的连续细胞系（JIII）的培养基中，检测到了蔗糖密度为1.18 - 1.22 g/cm³的细胞外颗粒。这些颗粒含有RNA、DNA和一种DNA聚合酶。它们以内源模板和引物合成DNA，也使用外源DNA作为模板，但不使用聚（rC）寡聚（dG）。用Nonidet P - 40预处理可刺激DNA聚合酶活性，而用核糖核酸酶处理则部分抑制该酶活性。针对这些颗粒制备的荧光抗体可使源自人类白血病的JIII和Z - 597细胞染色，但不能使测试的其他类型人类或非人类培养细胞染色。这些颗粒似乎不是肿瘤病毒，但可能是与造血和淋巴起源的恶性细胞相关的颗粒性抗原。