Mak T W, Manaster J, Howatson A F, McCulloch E A, Till J E
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4336-40. doi: 10.1073/pnas.71.11.4336.
An enzyme activity with the characteristics of RNA-directed DNA polymerase (reverse transcriptase) was detected in marrow from patients with leukemia in relapse and in firm hematological remission. Material having the enzyme activity, when analyzed in sucrose gradients, appeared as two distinct homogeneous bands of particles with densities of about 1.17 and 1.23 g/ml. The enzyme activity was stimulated by exogenous template poly(rC).(dG)(12-18) but not by (dT)(12-18). The enzyme activities in these bands also increased (1.7- to 24-fold) after culture, and both bands with enzyme activity were obtained from the cultured cells and from the supernatant medium. Electron microscopic studies showed that the two bands contained particles resembling leukoviruses or their cores.
在复发期白血病患者及血液学完全缓解患者的骨髓中检测到一种具有RNA指导的DNA聚合酶（逆转录酶）特性的酶活性。具有该酶活性的物质在蔗糖梯度中分析时，呈现为两条不同的均一颗粒带，密度分别约为1.17和1.23 g/ml。该酶活性受外源模板聚（rC）·（dG）（12 - 18）刺激，但不受（dT）（12 - 18）刺激。培养后这些条带中的酶活性也增加（1.7至24倍），并且两条具有酶活性的条带均来自培养细胞及上清培养基。电子显微镜研究表明，这两条带包含类似白血病病毒或其核心的颗粒。
