Sim Youn, Shin Seungwon
College of Pharmacy, Duksung Women's University, Ssangmoondong 419, Dobongku, Seoul, Korea.
Arch Pharm Res. 2008 Apr;31(4):497-502. doi: 10.1007/s12272-001-1184-7. Epub 2008 May 1.
The antifungal activities of ligustilide and butylidene phthalide, which are major oil components comprising the essential oil of Ligusticum chuanxiong, in combination with antifungal drugs were evaluated. Checkerboard microtiter and microdilution tests were used to measure combined effects against Trichophyton species, T. erinacei, T. mentagrophytes, T. rubrum, T. schoenleinii, T. tonsurans and T. soudanense. Susceptibility of these Trichophyton species to L. chuanxiong essential oil differed distinctly. The fractional inhibitory concentrations (FICIs) against Trichophyton species ranged between 0.27 and 0.56 for ketoconazole combined with ligustilde or butylidene phthalide, indicating synergism or additive effects between the antibiotic and essential oil compounds. Testing by itraconazole instead of ketoconazole, similar combination effects were observed with FICIs from 0.31 to 0.63 against the tested strains of T. mentagrophytes, T. rubrum, T. schoenleinii, and T. soudanense. Thus, data reported here showed that Trichophyton susceptibility to ketoconazole and itraconazole could be notably improved by ligustilde and butylidene phthalide.
评估了川芎挥发油的主要成分藁本内酯和丁烯基苯酞与抗真菌药物联合使用时的抗真菌活性。采用棋盘微量滴定法和微量稀释法测定其对毛癣菌属的联合作用，包括刺猬毛癣菌、须癣毛癣菌、红色毛癣菌、许兰毛癣菌、断发毛癣菌和苏丹毛癣菌。这些毛癣菌属对川芎挥发油的敏感性差异显著。酮康唑与藁本内酯或丁烯基苯酞联合使用时，对毛癣菌属的部分抑菌浓度（FICIs）在0.27至0.56之间，表明抗生素与挥发油成分之间存在协同或相加作用。用伊曲康唑代替酮康唑进行测试，对须癣毛癣菌、红色毛癣菌、许兰毛癣菌和苏丹毛癣菌测试菌株的FICIs在0.31至0.63之间，观察到类似的联合作用。因此，此处报告的数据表明，藁本内酯和丁烯基苯酞可显著提高毛癣菌对酮康唑和伊曲康唑的敏感性。
