Tang Zizhong, Qin Yihan, Chen Wenhui, Zhao Zhiqiao, Lin Wenjie, Xiao Yirong, Chen Hong, Liu Yuntao, Chen Hui, Bu Tongliang, Li Qingfeng, Cai Yi, Yao Huipeng, Wan Yujun
College of Life Sciences, Sichuan Agricultural University, Ya'an, China.
Sichuan Agricultural University Hospital, Ya'an, China.
Front Microbiol. 2021 Nov 17;12:771000. doi: 10.3389/fmicb.2021.771000. eCollection 2021.
The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. , sp., and sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of and sp. and the -butanol extract of sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 ± 0.006% and 90.11 ± 0.032%, respectively. At the same time, their IC values were only 0.18 ± 0.02 mg/mL and 0.04 ± 0.003 mg/mL. The ethyl acetate extract of showed MIC value of only 0.5 mg/mL against and . By liquid chromatography-mass spectrometry (LC-MS), we found that could produce many high-value polyphenols, such as hesperidin (36.06 μmol/g), ferulic acid (1.17 μmol/g), and alternariol (12.64 μmol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.
本研究的目的是评估霍尔特（CX）不同部位内生真菌的多样性，并进一步表征其生物活性，鉴定这些内生真菌产生的化合物。从CX中共分离鉴定出21株内生真菌。通过颜色反应，[具体真菌名称1]、[具体真菌名称2]和[具体真菌名称3]被鉴定为有潜力的菌株。比较这三株菌株的不同有机提取物，发现[具体真菌名称1]和[具体真菌名称2]的乙酸乙酯提取物以及[具体真菌名称3]的正丁醇提取物具有显著的抗氧化和抗菌活性。[具体真菌名称1]的乙酸乙酯提取物具有出色的抗氧化和抗菌效果，其对ABTS和DPPH的自由基清除率分别为98.43±0.006%和90.11±0.032%。同时，它们的IC值仅为0.18±0.02mg/mL和0.04±0.003mg/mL。[具体真菌名称1]的乙酸乙酯提取物对[具体细菌名称1]和[具体细菌名称2]的MIC值仅为0.5mg/mL。通过液相色谱-质谱联用（LC-MS），我们发现[具体真菌名称1]可以产生许多高价值的多酚类物质，如橙皮苷（36.06μmol/g）、阿魏酸（1.17μmol/g）和链格孢酚（12.64μmol/g），这可能成为制药行业的潜在资源。总之，这些结果增加了CX内生真菌的多样性及其次生代谢产物的抗氧化和抗菌活性。
