Khanal A, Yoshioka I, Tominaga K, Furuta N, Habu M, Fukuda J
Division of Maxillofacial Diagnostic and Surgical Science, Department of Oral and Maxillofacial Surgery, Kyushu Dental College,Kitakyushu, Japan.
Oral Dis. 2008 May;14(4):347-55. doi: 10.1111/j.1601-0825.2007.01388.x.
The aim of the present study was to clarify the mechanism of signal transduction of bone morphogenetic proteins (BMPs) through their specific down signaling molecules Smads inducing bone formation in response to mechanical stimulus during distraction osteogenesis (DO) in the rat mandible.
Osteotomy of the left mandible was performed in 45 rats. Thirty rats underwent mandibular distraction (protocol; 5 days latency, 8 days distraction, and 2 weeks consolidation) while 15 rats served as non-distracted (fracture healing) group. The expression of BMPs-2,-4 and Smads 1, 5, and 8 were evaluated in the new regenerate area using immunohistochemistry.
Expressions of BMPs-2,-4 and Smads 1, 5, and 8 were moderate during latency, significantly increased during distraction and decreased towards consolidation period.
The enhanced expression of BMPs and its Smads during distraction compared to the non-distracted group suggests the possible role of BMP signaling pathway in translation of mechanical forces into biological results during DO.
本研究旨在阐明骨形态发生蛋白（BMPs）通过其特定的下游信号分子Smads进行信号转导的机制，该机制在大鼠下颌骨牵张成骨（DO）过程中响应机械刺激诱导骨形成。
对45只大鼠进行左侧下颌骨截骨术。30只大鼠进行下颌骨牵张（方案：5天延迟期、8天牵张期和2周巩固期），而15只大鼠作为未牵张（骨折愈合）组。使用免疫组织化学方法评估新再生区域中BMPs-2、-4以及Smads 1、5和8的表达。
BMPs-2、-4以及Smads 1、5和8的表达在延迟期适中，在牵张期显著增加，而在巩固期降低。
与未牵张组相比，牵张期BMPs及其Smads的表达增强，这表明BMP信号通路在DO过程中将机械力转化为生物学结果中可能发挥作用。
