Lee Jeong-Eon, Seo Jae Pyo, Lee Dong Wan, Ko Young-Hwan, Lee Soon Dong
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2008 May;58(Pt 5):1084-8. doi: 10.1099/ijs.0.65541-0.
A novel actinomycete, designated strain LR-26T, was isolated from a small stone collected from an agricultural field in Jeju, Republic of Korea. Cells of the organism were strictly aerobic, Gram-positive, non-motile, short rods. Colonies were bright yellow, circular, smooth and translucent. The organism was characterized chemotaxonomically as having ll-diaminopimelic acid in the cell wall, MK-8(H4) as major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids, iso-C15 : 0 as the predominant fatty acid and a DNA G+C content of 72.6 mol%. Comparative 16S rRNA gene sequence analysis showed that the organism was related to the genera Intrasporangium, Terracoccus and Terrabacter within the family Intrasporangiaceae. The closest phylogenetic relatives of strain LR-26T were the type strains of Terrabacter terrae (99.4 % 16S rRNA gene sequence similarity), Terrabacter aerolatus (99.3 %) and Terrabacter tumescens (99.3 %). DNA-DNA hybridization experiments showed that strain LR-26T shared low levels of DNA-DNA relatedness with Terrabacter terrae LMG 22921T (17.6 and 22.8 % from reciprocal experiments) and with Terrabacter tumescens IMSNU 21313T (27.6 and 34.9 %). The phenotypic data and low levels of DNA-DNA relatedness readily distinguished strain LR-26T from the type strains of recognized species of the genus Terrabacter, and showed that it therefore represents a novel species. The name Terrabacter lapilli sp. nov. is proposed for this novel actinomycete. The type strain is LR-26T (=JBRI 2002T =KCTC 19199T =DSM 18583T).
从韩国济州岛一块农田采集的小石头中分离出一株新型放线菌，命名为LR-26T菌株。该菌细胞严格需氧，革兰氏阳性，不运动，呈短杆状。菌落亮黄色，圆形，光滑且半透明。该菌经化学分类学鉴定，细胞壁含ll-二氨基庚二酸，主要甲基萘醌为MK-8(H4)，极性脂质谱包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和未知磷脂，主要脂肪酸为异-C15 : 0，DNA G+C含量为72.6 mol%。16S rRNA基因序列比较分析表明，该菌与孢囊放线菌科的孢囊内孢菌属、土球菌属和土壤杆菌属相关。LR-26T菌株最接近的系统发育亲缘种是土生土壤杆菌模式菌株（16S rRNA基因序列相似性为99.4%）、气生土壤杆菌（99.3%）和肿胀土壤杆菌（99.3%）。DNA-DNA杂交实验表明，LR-26T菌株与土生土壤杆菌LMG 22921T（双向实验分别为17.6%和22.8%）以及肿胀土壤杆菌IMSNU 21313T（双向实验分别为27.6%和34.9%）的DNA-DNA相关性较低。表型数据和低水平的DNA-DNA相关性很容易将LR-26T菌株与土壤杆菌属已确认种的模式菌株区分开来，表明它代表一个新物种。为此新型放线菌提议命名为土生土壤杆菌新种（Terrabacter lapilli sp. nov.）。模式菌株为LR-26T（=JBRI 2002T =KCTC 19199T =DSM 18583T）。
