Onose Jun-Ichi, Xie Chun, Ye Yue Qi, Sugaya Kouichi, Takahashi Shunya, Koshino Hiroyuki, Yasunaga Katsuaki, Abe Naoki, Yoshikawa Kunie
Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Biol Pharm Bull. 2008 May;31(5):831-3. doi: 10.1248/bpb.31.831.
Thelephora vialis is a mushroom that grows in symbiosis with pine trees in Yunnan, China, a place known to have some of the richest and most diverse bioresources in the world. This is one of the most favored edible mushrooms, due to its flavor. Our screening for bioactive compounds from these mushrooms isolated a novel potent antioxidant, vialinin A, together with known compounds, from the dry fruiting bodies of T. vialis. Vialinin A is a terphenyl derivative and was elucidated by spectroscopic and chemical methods. Vialinin A showed anti-allergic activities, inhibition of beta-hexosaminidase, tumor necrosis factor (TNF)-alpha, interleukin 4 and monocyte chemotactic protein 1 release from RBL-2H3 cells, whereas atromentin and an inseparable mixture of ganbajunins D and E showed no such effects. Vialinin A displayed potent inhibition of TNF-alpha production from RBL-2H3 cells (IC50, 0.09+/-0.01 nM), indicating stronger inhibition than tacrolimus for organ transplantation (IC50, 0.25+/-0.03 nM). The potent inhibitory activities of these compounds against TNF-alpha production indicate promising new candidates for anti-allergic agents.
绿孢地卷是一种与中国云南松树共生生长的蘑菇，云南是世界上生物资源最丰富、最多样化的地区之一。由于其风味，它是最受欢迎的食用菌之一。我们从这些蘑菇中筛选生物活性化合物，从绿孢地卷的干燥子实体中分离出一种新型强效抗氧化剂——绿孢菌素A以及一些已知化合物。绿孢菌素A是一种三联苯衍生物，通过光谱和化学方法对其进行了鉴定。绿孢菌素A表现出抗过敏活性，能够抑制β-己糖胺酶、肿瘤坏死因子（TNF）-α、白细胞介素4以及单核细胞趋化蛋白1从RBL-2H3细胞中的释放，而栓菌酸以及无法分离的干巴菌宁D和E的混合物则没有这种作用。绿孢菌素A对RBL-2H3细胞产生的TNF-α具有强效抑制作用（IC50，0.09±0.01 nM），表明其抑制作用比用于器官移植的他克莫司更强（IC50，0.25±0.03 nM）。这些化合物对TNF-α产生的强效抑制活性表明它们有望成为抗过敏药物的新候选物。
